Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Gallus Malectin (MLEC) Polyclonal Antibody | anti-MLEC antibody
Polyclonal Antibody to Malectin (MLEC)
Gene Names
MLEC; KIAA0152
Reactivity
Gallus
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Malectin (MLEC); Polyclonal Antibody; Polyclonal Antibody to Malectin (MLEC); anti-MLEC antibody
Host
Rabbit
Reactivity
Gallus
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against MLEC. It has been selected for its ability to recognize MLEC in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with a His-tag and its sequence is listed below.
MGHHHHHHSGSEF-LLTTDKPEE KMYSCPIIQC SAPAVNGLPG RDGRDGPKGE KGDPGEGLRG LQGLPGKAGP QGLKGEVGPQ GEKGQKGERG IVVTDDLHRQ ITDLEAKIRV LEDDLSRYKK ALSLKDVVNV GKKMFVSTGK KYNFEKGKSL CAKAGSVLAS PRNEAENTAL KDLIDPSSQA YIGISDAQTE GRFMYLSGGP LTYSNWKPGE PNNHKNEDCA VIEDSGKWND LDCSNSNIFI ICEL
MGHHHHHHSGSEF-LLTTDKPEE KMYSCPIIQC SAPAVNGLPG RDGRDGPKGE KGDPGEGLRG LQGLPGKAGP QGLKGEVGPQ GEKGQKGERG IVVTDDLHRQ ITDLEAKIRV LEDDLSRYKK ALSLKDVVNV GKKMFVSTGK KYNFEKGKSL CAKAGSVLAS PRNEAENTAL KDLIDPSSQA YIGISDAQTE GRFMYLSGGP LTYSNWKPGE PNNHKNEDCA VIEDSGKWND LDCSNSNIFI ICEL
Sequence Length
292
Applicable Applications for anti-MLEC antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant MLEC (Leu22~Leu254) expressed in E.coli.
Cross Reactivity
Chicken
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2084000
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
32,234 Da
NCBI Official Full Name
malectin
NCBI Official Synonym Full Names
malectin
NCBI Official Symbol
MLEC
NCBI Official Synonym Symbols
KIAA0152
NCBI Protein Information
malectin; oligosaccharyltransferase complex subunit (non-catalytic)
UniProt Protein Name
Malectin
Protein Family
UniProt Gene Name
MLEC
UniProt Synonym Gene Names
KIAA0152
UniProt Entry Name
MLEC_HUMAN
Uniprot Description
MLEC: Carbohydrate-binding protein with a strong ligand preference for Glc2-N-glycan. May play a role in the early steps of protein N-glycosylation. Belongs to the malectin family.
Protein type: Membrane protein, integral; Endoplasmic reticulum
Chromosomal Location of Human Ortholog: 12q24.31
Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane
Molecular Function: enzyme binding; carbohydrate binding
Biological Process: cellular protein metabolic process; protein folding; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification
Research Articles on MLEC
Similar Products
Product Notes
The MLEC mlec (Catalog #AAA2006581) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Malectin (MLEC) reacts with Gallus and may cross-react with other species as described in the data sheet. AAA Biotech's Malectin (MLEC) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the MLEC mlec for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with a His-tag and its sequence is listed below. MGHHHHHHSG SEF-LLTTDK PEE KMYSCPIIQC SAPAVNGLPG RDGRDGPKGE KGDPGEGLRG LQGLPGKAGP QGLKGEVGPQ GEKGQKGERG IVVTDDLHRQ ITDLEAKIRV LEDDLSRYKK ALSLKDVVNV GKKMFVSTGK KYNFEKGKSL CAKAGSVLAS PRNEAENTAL KDLIDPSSQA YIGISDAQTE GRFMYLSGGP LTYSNWKPGE PNNHKNEDCA VIEDSGKWND LDCSNSNIFI ICEL. It is sometimes possible for the material contained within the vial of "Malectin (MLEC), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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