PMVK cdna clone

PMVK

Cat. Num. AAA200641

• Gene Names: PMVK; PMK; PMKA; PMKASE; POROK1; HUMPMKI
• Synonyms: PMVK; HUMPMKI; PMK; PMKA; PMKASE; PMVK cdna clone

• Form/Format: Lyophilized
• Sequence: Nucleotide Sequence: ATGGCCCCGCTGGGAGGCGCCCCGCGGCTGGTACTGCTGTTCAGCGGCAAGAGGAAATCCGGGAAGGACTTCGTGACCGAGGCGCTGCAGAGCAGACTTGGAGCTGATGTCTGTGCTGTCCTCCGGCTCTCTGGTCCACTCAAGGAACAGTATGCTCAGGAGCATGGCTTGAACTTCCAGAGACTCCTGGACACCAGCACCTACAAGGAGGCCTTTCGGAAGGACATGATCCGCTGGGGAGAGGAGAAACGCCAGGCTGACCCAGGCTTCTTTTGCAGGAAGATTGTGGAGGGCATCTCCCAGCCCATCTGGCTGGTGAGTGACACACGGAGAGTGTCTGACATCCAGTGGTTTCGGGAGGCCTATGGGGCCGTGACGCAGACGGTCCGCGTTGTAGCGTTGGAGCAGAGCCGACAGCAGCGGGGCTGGGTGTTCACGCCAGGGGTGGACGATGCTGAGTCAGAATGTGGCCTGGACAACTTCGGGGACTTTGACTGGGTCATCGAGAACCATGGAGTTGAACAGCGCCTGGAGGAGCAGTTGGAGAACCTGATAGAATTTATCCGCTCCAGACTTTAG; ; Translation Sequence: MAPLGGAPRL VLLFSGKRKS GKDFVTEALQ SRLGADVCAV LRLSGPLKEQ YAQEHGLNFQ RLLDTSTYKE AFRKDMIRWG EEKRQADPGF FCRKIVEGIS QPIWLVSDTR RVSDIQWFRE AYGAVTQTVR VVALEQSRQQ RGWVFTPGVD DAESECGLDN FGDFDWVIEN HGVEQRLEEQ LENLIEFIRS RL
• Sequence Length: 192

• Species: Human
• Chromosome Location: 1q22
• OMIM Reference Number: 607622
• cDNA Size: 579bp
• Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
• Vector: (puc19-derived cloning vector)
• Preparation Before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid.
• Preparation and Storage: Store the plasmid at -20 degree C.

Related Product Information for PMVK cdna clone

PMVK encodes a peroxisomal enzyme that catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate, the fifth reaction of the cholesterol biosynthetic pathway. Studies in rat show that the message level and the enzyme activity of this protein is regulated by sterol, and that this regulation is coordinated with 3-hydroxy-3-methylglutaryl coenzyme A reductase, the rate-limiting enzyme of cholesterol biosynthesis.

Product Categories/Family for PMVK cdna clone

Metabolism; Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 5729980
• NCBI GeneID: 10654
• NCBI Accession #: NP_006547.1
• NCBI GenBank Nucleotide #: NM_006556.3
• UniProt Accession #: Q15126
• NCBI Official Full Name: phosphomevalonate kinase isoform 1
• NCBI Official Synonym Full Names: phosphomevalonate kinase
• NCBI Official Symbol: PMVK
• NCBI Official Synonym Symbols: PMK; PMKA; PMKASE; POROK1; HUMPMKI
• NCBI Protein Information: phosphomevalonate kinase
• UniProt Protein Name: Phosphomevalonate kinase
• UniProt Gene Name: PMVK
• UniProt Synonym Gene Names: PMKI; PMKase; hPMK
• UniProt Entry Name: PMVK_HUMAN

NCBI Description

This gene encodes a peroxisomal enzyme that is a member of the galactokinase, homoserine kinase, mevalonate kinase, and phosphomevalonate kinase (GHMP) family of ATP-dependent enzymes. The encoded protein catalyzes the conversion of mevalonate 5-phosphate to mevalonate 5-diphosphate, which is the fifth step in the mevalonate pathway of isoprenoid biosynthesis. Mutations in this gene are linked to certain types of porokeratosis including disseminated superficial porokeratosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2017]

Uniprot Description

PMVK: Induced by sterol. Monomer.

Protein type: Kinase, other; EC 2.7.4.2; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: membrane; peroxisome; cytosol

Molecular Function: phosphomevalonate kinase activity; ATP binding

Biological Process: isopentenyl diphosphate biosynthetic process, mevalonate pathway; sterol biosynthetic process; phosphorylation; cholesterol biosynthetic process