Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Semaphorin 3E (SEMA3E) Polyclonal Antibody | anti-SEMA3E antibody
Polyclonal Antibody to Semaphorin 3E (SEMA3E)
Gene Names
Sema3e; Semah; AA408817; mKIAA0331; 6430702L12
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Semaphorin 3E (SEMA3E); Polyclonal Antibody; Polyclonal Antibody to Semaphorin 3E (SEMA3E); anti-SEMA3E antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against SEMA3E. It has been selected for its ability to recognize SEMA3E in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with a His-tag and its sequence is listed below.
MGHHHHHHSGSEF-ALDRTEERLA YGIESNSTLL ECTPRSLQAK VIWFVQKGRD VRKEEVKTDD RVVKMDLGLL FLRVRKSDAG TYFCQTVEHN FVHTVRKIT
MGHHHHHHSGSEF-ALDRTEERLA YGIESNSTLL ECTPRSLQAK VIWFVQKGRD VRKEEVKTDD RVVKMDLGLL FLRVRKSDAG TYFCQTVEHN FVHTVRKIT
Sequence Length
775
Applicable Applications for anti-SEMA3E antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant SEMA3E (Ala581~Thr669) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2083414
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Lung tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Lung tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
89,543 Da
NCBI Official Full Name
semaphorin-3E
NCBI Official Synonym Full Names
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E
NCBI Official Symbol
Sema3e
NCBI Official Synonym Symbols
Semah; AA408817; mKIAA0331; 6430702L12
NCBI Protein Information
semaphorin-3E
UniProt Protein Name
Semaphorin-3E
Protein Family
UniProt Gene Name
Sema3e
UniProt Synonym Gene Names
Semah; Semh; Sema H
Uniprot Description
SEMA3E: a secreted protein of the semaphorin family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 5|5 A1
Cellular Component: extracellular region; extracellular space
Molecular Function: protein binding; semaphorin receptor binding
Biological Process: angiogenesis; branching involved in blood vessel morphogenesis; cell differentiation; multicellular organism development; negative chemotaxis; negative regulation of angiogenesis; negative regulation of cell-matrix adhesion; nervous system development; regulation of cell shape; sprouting angiogenesis; synapse organization
Research Articles on SEMA3E
Similar Products
Product Notes
The SEMA3E sema3e (Catalog #AAA2005529) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Semaphorin 3E (SEMA3E) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Semaphorin 3E (SEMA3E) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the SEMA3E sema3e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with a His-tag and its sequence is listed below. MGHHHHHHSG SEF-ALDRTE ERLA YGIESNSTLL ECTPRSLQAK VIWFVQKGRD VRKEEVKTDD RVVKMDLGLL FLRVRKSDAG TYFCQTVEHN FVHTVRKIT. It is sometimes possible for the material contained within the vial of "Semaphorin 3E (SEMA3E), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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