Typical Testing Data/Standard Curve (for reference only)
Mouse Semaphorin 3E (SEMA3E) ELISA Kit | SEMA3E elisa kit
Mouse Semaphorin 3E (SEMA3E) ELISA Kit
Gene Names
Sema3e; Semah; AA408817; mKIAA0331; 6430702L12
Reactivity
Mouse
Synonyms
Semaphorin 3E (SEMA3E); Mouse Semaphorin 3E (SEMA3E) ELISA Kit; SEMAH; M-SemaK; M-sema H; coll-5; Sema Domain; Immunoglobulin Domain(Ig); Transmembrane Domain(TM)and Short Cytoplasmic Domain 3E; SEMA3E elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Semaphorin 3E (SEMA3E).
No significant cross-reactivity or interference between Semaphorin 3E (SEMA3E) and analogues was observed.
No significant cross-reactivity or interference between Semaphorin 3E (SEMA3E) and analogues was observed.
Sequence Length
775
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates, Cell lysates and Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.059ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Semaphorin 3E (SEMA3E) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Semaphorin 3E (SEMA3E) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SEMA3E elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 3E (SEMA3E). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semaphorin 3E (SEMA3E). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semaphorin 3E (SEMA3E), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Semaphorin 3E (SEMA3E) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for SEMA3E elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
89,543 Da
NCBI Official Full Name
semaphorin-3E
NCBI Official Synonym Full Names
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E
NCBI Official Symbol
Sema3e
NCBI Official Synonym Symbols
Semah; AA408817; mKIAA0331; 6430702L12
NCBI Protein Information
semaphorin-3E
UniProt Protein Name
Semaphorin-3E
Protein Family
UniProt Gene Name
Sema3e
UniProt Synonym Gene Names
Semah; Semh; Sema H
UniProt Entry Name
SEM3E_MOUSE
Uniprot Description
SEMA3E: a secreted protein of the semaphorin family.
Protein type: Secreted; Secreted, signal peptide
Cellular Component: membrane; extracellular region
Molecular Function: protein binding; receptor activity; semaphorin receptor binding
Biological Process: patterning of blood vessels; regulation of cell shape; nervous system development; negative regulation of angiogenesis; multicellular organismal development; synapse organization and biogenesis; sprouting angiogenesis; angiogenesis; negative regulation of cell-matrix adhesion; cell differentiation
Research Articles on SEMA3E
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Product Notes
The Mouse SEMA3E sema3e (Catalog #AAA2024010) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2024010 ELISA Kit recognizes Mouse SEMA3E. It is sometimes possible for the material contained within the vial of "Semaphorin 3E (SEMA3E), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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