Immunocytochemistry/Immunofluorescence (ICC/IF)
(ICC/IF analysis of Cyclophilin B in Hep3B cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Mouse anti-Human Cyclophilin B Monoclonal Antibody | anti-PPIB antibody
Cyclophilin B
Immunocytochemistry/Immunofluorescence (ICC/IF)
(ICC/IF analysis of Cyclophilin B in Hep3B cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Immunocytochemistry/Immunofluorescence (ICC/IF)
(ICC/IF analysis of Cyclophilin B in Hep3B cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclophilin B antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HepG2 cell lysate;;Lane 2.: HeLa cell lysate;;Lane 3.: Jurkate cell lysate;;Lane 4.: K562 cell lysate;;Lane 5.: 293T cell lysate;;Lane 6.: A549 cell lysate;;Lane 7.: MCF7 cell lysate;;Lane 8.: sk-ov-3 cell lysate;;Lane 9.: LnCap cell lysate)
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclophilin B antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HepG2 cell lysate;;Lane 2.: HeLa cell lysate;;Lane 3.: Jurkate cell lysate;;Lane 4.: K562 cell lysate;;Lane 5.: 293T cell lysate;;Lane 6.: A549 cell lysate;;Lane 7.: MCF7 cell lysate;;Lane 8.: sk-ov-3 cell lysate;;Lane 9.: LnCap cell lysate)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Variants have been identified in this protein that give rise to recessive forms of osteogenesis imperfecta. [provided by RefSeq, Oct 2009]
Uniprot Description
PPIB: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Defects in PPIB are the cause of osteogenesis imperfecta type 9 (OI9). OI9 is a connective tissue disorder characterized by bone fragility, low bone mass and bowing of limbs due to multiple fractures. Short limb dwarfism and blue sclerae are observed in some but not all patients. Belongs to the cyclophilin-type PPIase family. PPIase B subfamily.
Protein type: Isomerase; EC 5.2.1.8; Chaperone; Cyclophilin; Secreted, signal peptide; RNA-binding; Secreted
Chromosomal Location of Human Ortholog: 15q21-q22
Cellular Component: smooth endoplasmic reticulum; focal adhesion; membrane; perinuclear region of cytoplasm; endoplasmic reticulum lumen; endoplasmic reticulum; melanosome; nucleus
Molecular Function: collagen binding; protein binding; peptidyl-prolyl cis-trans isomerase activity; unfolded protein binding; protein complex binding; peptide binding
Biological Process: extracellular matrix organization and biogenesis; protein peptidyl-prolyl isomerization; protein stabilization; positive regulation of multicellular organism growth
Disease: Osteogenesis Imperfecta, Type Ix
Research Articles on PPIB
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Product Notes
The PPIB ppib (Catalog #AAA200508) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Cyclophilin B reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cyclophilin B can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Researchers should empirically determine the suitability of the PPIB ppib for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cyclophilin B, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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