Rabbit anti-Human PPAP2C Polyclonal Antibody | anti-PPAP2C antibody

PPAP2C, CT (Lipid Phosphate Phosphohydrolase 2, Phosphatidic Acid Phosphatase 2c, PAP-2c, PAP2c, Phosphatidate Phosphohydrolase Type 2c, PAP2-gamma, PAP2-G, LPP2) (MaxLight 550)

Cat. Num. AAA6492986

• Gene Names: PPAP2C; LPP2; PAP-2c; PAP2-g
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: PPAP2C; Polyclonal Antibody; CT (Lipid Phosphate Phosphohydrolase 2; Phosphatidic Acid Phosphatase 2c; PAP-2c; PAP2c; Phosphatidate Phosphohydrolase Type 2c; PAP2-gamma; PAP2-G; LPP2) (MaxLight 550); anti-PPAP2C antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PPAP2C.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-PPAP2C antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human PPAP2C (KLH).
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-PPAP2C Antibody (C-term) in NCI-H460 cell line lysates (35ug/lane).PPAP2C(arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(PPAP2C Antibody (C-term) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-PPAP2C antibody

Antibodies; Enzymes; Phosphatase

References

1. Long, J.S., Biochem. J. 411(2), 371-377 (2008). 2. Morris, K.E., J. Biol. Chem. 281(14), 9297-9306 (2006). 3. Nanjundan, M., Am. J. Physiol. Lung Cell Mol. Physiol. 284(1), L1-L23 (2003). 4. Zhang, N., Genesis 27(4), 137-140 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 4505977
• NCBI GeneID: 8612
• NCBI Accession #: NP_003703
• NCBI GenBank Nucleotide #: NM_003712.3
• UniProt Accession #: O43688; A6NLV0; E9PAY8
• Molecular Weight: SDS-PAGE MW: 33kd
• NCBI Official Full Name: lipid phosphate phosphohydrolase 2 isoform 1
• NCBI Official Synonym Full Names: phosphatidic acid phosphatase type 2C
• NCBI Official Symbol: PPAP2C
• NCBI Official Synonym Symbols: LPP2; PAP-2c; PAP2-g
• NCBI Protein Information: lipid phosphate phosphohydrolase 2
• UniProt Protein Name: Lipid phosphate phosphohydrolase 2
• UniProt Gene Name: PPAP2C
• UniProt Synonym Gene Names: LPP2; PAP2-G; PAP-2c; PAP2c
• UniProt Entry Name: LPP2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the phosphatidic acid phosphatase (PAP) family. PAPs convert phosphatidic acid to diacylglycerol, and function in de novo synthesis of glycerolipids as well as in receptor-activated signal transduction mediated by phospholipase D. This protein is similar to phosphatidic acid phosphatase type 2A (PPAP2A) and type 2B (PPAP2B). All three proteins contain 6 transmembrane regions, and a consensus N-glycosylation site. This protein has been shown to possess membrane associated PAP activity. Three alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Catalyzes the conversion of phosphatidic acid (PA) to diacylglycerol (DG). In addition it hydrolyzes lysophosphatidic acid (LPA), ceramide-1-phosphate (C-1-P) and sphingosine-1-phosphate (S-1-P). The relative catalytic efficiency is PA > C-1-P > LPA > S-1-P.

Catalytic activity: A 1,2-diacylglycerol 3-phosphate + H2O = a 1,2-diacyl-sn-glycerol + phosphate.

Enzyme regulation: Inhibited by sphingosine, zinc ions and propanolol.

Subunit structure: Homodimer

By similarity.

Subcellular location: Membrane; Multi-pass membrane protein.

Tissue specificity: Found mainly in brain, pancreas and placenta.

Sequence similarities: Belongs to the PA-phosphatase related phosphoesterase family.