Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Kidney Tissue; Lane2: Mouse Placenta Tissue.)
Rabbit anti-Mouse Collagen Type I Alpha 2 (COL1a2) Polyclonal Antibody | anti-COL1a2 antibody
Polyclonal Antibody to Collagen Type I Alpha 2 (COL1a2)
MGHHHHHHSGSEF-ATYRLNYT RTSLDVGTET TWEVKVVARI RPATDTGVLL ALVGDDDVVP ISVALVDYHS TKKLKKQLVV LAVEDVALAL MEIKVCDSQE HTVTVSLREG EATLEVDGTK GQSEVSTAQL QERLDTLKTH LQGSVHTYVG GLPEVSVISA PVTAFYRGCM TLEVNG
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Kidney Tissue; Lane2: Mouse Placenta Tissue.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Kidney Tissue; Lane2: Mouse Placenta Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant COL1a2, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant COL1a2, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Spleen Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Spleen Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Spleen Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Spleen Tissue))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the alpha-2 subunit of the fibril-forming type I collagen, the most abundant protein of bone, skin and tendon extracellular matrices. The encoded protein, in association with alpha-1 subunit, forms heterotrimeric type I procollagen that undergoes proteolytic processing during fibril formation. Mice harboring certain mutations in the encoded gene exhibit symptoms of moderate to severe forms of osteogenesis imperfecta. [provided by RefSeq, Dec 2015]
Uniprot Description
COL1A2: Type I collagen is a member of group I collagen (fibrillar forming collagen). Defects in COL1A2 are the cause of Ehlers-Danlos syndrome type 7B (EDS7B). EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7B is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations. Defects in COL1A2 are a cause of osteogenesis imperfecta type 1 (OI1). A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta. Defects in COL1A2 are a cause of osteogenesis imperfecta type 2 (OI2); also known as osteogenesis imperfecta congenita (OIC) or lethal perinatal. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency. Defects in COL1A2 are the cause of Ehlers-Danlos syndrome autosomal recessive cardiac valvular form (EDSCV). A connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. In addition to joint laxity, skin hyperextensibility and friability, and abnormal scar formation, patients have mitral valve prolapse and insufficiency, mitral regurgitation, and aortic insufficiency. Defects in COL1A2 are a cause of osteogenesis imperfecta type 3 (OI3). A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta. Defects in COL1A2 are a cause of osteogenesis imperfecta type 4 (OI4); also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta. A chromosomal aberration involving COL1A2 may be a cause of lipoblastomas, which are benign tumors resulting from transformation of adipocytes, usually diagnosed in children. Translocation t(7;8)(p22;q13) with PLAG1. Belongs to the fibrillar collagen family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 6 A1|6 1.81 cM
Cellular Component: collagen; collagen type I trimer; endoplasmic reticulum; extracellular matrix; extracellular region; extracellular space; proteinaceous extracellular matrix
Molecular Function: extracellular matrix structural constituent; identical protein binding; metal ion binding; platelet-derived growth factor binding; protease binding; protein binding; protein binding, bridging; SMAD binding
Biological Process: blood vessel development; collagen fibril organization; regulation of blood pressure; Rho protein signal transduction; skeletal system development; skin morphogenesis; transforming growth factor beta receptor signaling pathway
Research Articles on COL1a2
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Product Notes
The COL1a2 col1a2 (Catalog #AAA2004986) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Collagen Type I Alpha 2 (COL1a2) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Collagen Type I Alpha 2 (COL1a2) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the COL1a2 col1a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-ATYRLN YT RTSLDVGTET TWEVKVVARI RPATDTGVLL ALVGDDDVVP ISVALVDYHS TKKLKKQLVV LAVEDVALAL MEIKVCDSQE HTVTVSLREG EATLEVDGTK GQSEVSTAQL QERLDTLKTH LQGSVHTYVG GLPEVSVISA PVTAFYRGCM TLEVNG. It is sometimes possible for the material contained within the vial of "Collagen Type I Alpha 2 (COL1a2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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