Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using ASK1 antibody)
Rabbit ASK1 Polyclonal Antibody | anti-ASK1 antibody
ASK1 antibody
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified by immunoaffinity chromatography.
Synonyms
ASK1; Polyclonal Antibody; ASK1 antibody; Rabbit polyclonal ASK1 antibody; Polyclonal ASK1 antibody; Anti-ASK1 antibody; MAP3K5 antibody; ASK-1 antibody; Mitogen-activated protein kinase kinase kinase 5 antibody; Apoptosis signal-regulating kinase 1 antibody; Ab 83 antibody; ASK 1 antibody; ASK-1; ASK 1; anti-ASK1 antibody
Host
Rabbit
Clonality
Polyclonal
Purity/Purification
Purified by immunoaffinity chromatography.
Form/Format
Liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl,0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
472
Applicable Applications for anti-ASK1 antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IF: 1:100-1:200
IHC: 1:50-1:100
WB: 1:500-1:1000
IHC: 1:50-1:100
WB: 1:500-1:1000
Immunogen
ASK1 antibody was raised in Rabbit using the peptide sequence around aa 81~85 (G-S-S-V-G) derived from Human ASK1 as the immunogen.
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage.
Aliquot and store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.
Aliquot and store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using ASK1 antibody)
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using ASK1 antibody)
Western Blot (WB)
(Western Blot analysis of extracts from MDA-MB-435 cells using ASK1 antibody and the same antibody preincubated with blocking peptide.)
Western Blot (WB)
(Western Blot analysis of extracts from MDA-MB-435 cells using ASK1 antibody and the same antibody preincubated with blocking peptide.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ASK1 antibody (left) or the same antibody preincubated with with blocking peptide (right))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ASK1 antibody (left) or the same antibody preincubated with with blocking peptide (right))
Related Product Information for anti-ASK1 antibody
ASK1 is a member of MAP kinase kinase kinase family and as such a part of mitogen-activated protein kinase pathway. It activates c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinases in a Raf-independent fashion in response to an array of stresses such as oxidative stress, endoplasmic reticulum stress and calcium influx. ASK1 has been found to be involved in cancer, diabetes, cardiovascular and neurodegenerative diseases.
Product Categories/Family for anti-ASK1 antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
52,534 Da
NCBI Official Full Name
ASK1
UniProt Protein Name
ASK1 protein
Protein Family
UniProt Gene Name
ASK1
Similar Products
Product Notes
The ASK1 ask1 (Catalog #AAA5309720) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ASK1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). IF: 1:100-1:200 IHC: 1:50-1:100 WB: 1:500-1:1000. Researchers should empirically determine the suitability of the ASK1 ask1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ASK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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