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Western Blot (WB) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human S100A11 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : MCF7 cell lysate Lane 3. : PC3 cell lysate Lane 4. : HaCaT cell lysate Lane 5. : THP1 cell lysate Lane 6. : U87MG cell lysate Lane 7. : A427 cell lysate Lane 8. : SK-OV-3 cell lysate Lane 9. : A549 cell lysate)

Mouse anti-Human S100A11 Monoclonal Antibody | anti-S100A11 antibody

S100A11 antibody

Gene Names
S100A11; MLN70; S100C; HEL-S-43
Reactivity
Human
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence
Purity
By protein-A affinity chromatography
Synonyms
S100A11; Monoclonal Antibody; S100A11 antibody; S100 calcium binding protein A11; MLN70; S100C; anti-S100A11 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT20D11
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
105
Applicable Applications for anti-S100A11 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Antigen Species
Human
Immunogen
Recombinant human S100A11 (1-105aa) purified from E. coli
Clone Note
Anti-human S100A11 mAb, clone AT20D11, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human S100A11 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.

Western Blot (WB)

(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human S100A11 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : MCF7 cell lysate Lane 3. : PC3 cell lysate Lane 4. : HaCaT cell lysate Lane 5. : THP1 cell lysate Lane 6. : U87MG cell lysate Lane 7. : A427 cell lysate Lane 8. : SK-OV-3 cell lysate Lane 9. : A549 cell lysate)

Western Blot (WB) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human S100A11 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : MCF7 cell lysate Lane 3. : PC3 cell lysate Lane 4. : HaCaT cell lysate Lane 5. : THP1 cell lysate Lane 6. : U87MG cell lysate Lane 7. : A427 cell lysate Lane 8. : SK-OV-3 cell lysate Lane 9. : A549 cell lysate)

Immunofluorescence (IF)

(ICC/IF analysis of S100A11 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human S100A11 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)

Immunofluorescence (IF) (ICC/IF analysis of S100A11 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human S100A11 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Related Product Information for anti-S100A11 antibody
S100A11 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. This protein may function in motility, invasion, and tubulin polymerization.
Product Categories/Family for anti-S100A11 antibody
References
Sakaguchi M., et al. (2003) J Cell Biol. 163: 825-35.; Settmacher U., et al. (2005) Int. J. Oncol. 28: 195-200.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
11,740 Da
NCBI Official Full Name
protein S100-A11
NCBI Official Synonym Full Names
S100 calcium binding protein A11
NCBI Official Symbol
S100A11
NCBI Official Synonym Symbols
MLN70; S100C; HEL-S-43
NCBI Protein Information
protein S100-A11
UniProt Protein Name
Protein S100-A11
Protein Family
UniProt Gene Name
S100A11
UniProt Synonym Gene Names
MLN70; S100C; MLN 70

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. [provided by RefSeq, Jul 2008]

Uniprot Description

Facilitates the differentiation and the cornification of keratinocytes.

Research Articles on S100A11

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Product Notes

The S100A11 s100a11 (Catalog #AAA200440) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The S100A11 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's S100A11 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the S100A11 s100a11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "S100A11, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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