Western Blot (WB)
(Western Blot: Sample: Rat Heart lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat CX3CL1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Rat Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) Polyclonal Antibody | anti-CX3CL1 antibody
Polyclonal Antibody to Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)
Gene Names
Cx3cl1; Cx3c; Scyd1
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Chemokine C-X3-C-Motif Ligand 1 (CX3CL1); Polyclonal Antibody; Polyclonal Antibody to Chemokine C-X3-C-Motif Ligand 1 (CX3CL1); anti-CX3CL1 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against CX3CL1. It has been selected for its ability to recognize CX3CL1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-WVQDAMKHLD HQTAALTRNG GKFEKRVDNV TPRITSATRG LSPTALAKPE SATVEDLTLE PTAISQEARR PMGTSQEPPA AVTGSSPSTS KAQDAGLAAK PQSTGISEVA AVSTTIWPSS AVYQSGSSLW AEEKATESPP TIALSTQAST TSSPKQNVGS EGQPPWVQEQ DSTPEKSPGP EETNPVHTDI FQDRGPGSTV HPSVAPTSSE KTPSPELVASGSQAPKVEEP IHATADPQKL SVFITPVPDS QAATRRQ
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-WVQDAMKHLD HQTAALTRNG GKFEKRVDNV TPRITSATRG LSPTALAKPE SATVEDLTLE PTAISQEARR PMGTSQEPPA AVTGSSPSTS KAQDAGLAAK PQSTGISEVA AVSTTIWPSS AVYQSGSSLW AEEKATESPP TIALSTQAST TSSPKQNVGS EGQPPWVQEQ DSTPEKSPGP EETNPVHTDI FQDRGPGSTV HPSVAPTSSE KTPSPELVASGSQAPKVEEP IHATADPQKL SVFITPVPDS QAATRRQ
Sequence Length
397
Applicable Applications for anti-CX3CL1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant CX3CL1 (Trp81~Gln337) expressed in E.coli.
Cross Reactivity
Human, Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2035757
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Rat Heart lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat CX3CL1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Rat Heart lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat CX3CL1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Rat Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Rat Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
41,977 Da
NCBI Official Full Name
fractalkine
NCBI Official Synonym Full Names
C-X3-C motif chemokine ligand 1
NCBI Official Symbol
Cx3cl1
NCBI Official Synonym Symbols
Cx3c; Scyd1
NCBI Protein Information
fractalkine
UniProt Protein Name
Fractalkine
Protein Family
UniProt Gene Name
Cx3cl1
UniProt Synonym Gene Names
Acc1; Fkn; Scyd1
NCBI Description
chemokine that binds its receptor CX3CR1; induces chemotaxis and increased intracellular calcium levels in microglia [RGD, Feb 2006]
Uniprot Description
Acts as a ligand for both CX3CR1 and integrins. Binds to CX3CR1 and to integrins ITGAV:ITGB3 and ITGA4:ITGB1. Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner. In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins. In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1. The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium.
Research Articles on CX3CL1
Similar Products
Product Notes
The CX3CL1 cx3cl1 (Catalog #AAA2003539) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the CX3CL1 cx3cl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF -WVQDAMKHL D HQTAALTRNG GKFEKRVDNV TPRITSATRG LSPTALAKPE SATVEDLTLE PTAISQEARR PMGTSQEPPA AVTGSSPSTS KAQDAGLAAK PQSTGISEVA AVSTTIWPSS AVYQSGSSLW AEEKATESPP TIALSTQAST TSSPKQNVGS EGQPPWVQEQ DSTPEKSPGP EETNPVHTDI FQDRGPGSTV HPSVAPTSSE KTPSPELVAS GSQAPKVEEP IHATADPQKL SVFITPVPDS QAATRRQ. It is sometimes possible for the material contained within the vial of "Chemokine C-X3-C-Motif Ligand 1 (CX3CL1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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