Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) Recombinant Protein | CX3CL1 recombinant protein

Recombinant Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)

Cat. Num. AAA2009145

• Gene Names: Cx3cl1; Cx3c; Scyd1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Chemokine C-X3-C-Motif Ligand 1 (CX3CL1); Recombinant Chemokine C-X3-C-Motif Ligand 1 (CX3CL1); CX3CL1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSG SEF- WVQDAMKHLD HQTAALTRNG GKFEKRVDNV TPRITSATRG LSPTALAKPE SATVEDLTLE PTAISQEARR PMGTSQEPPA AVTGSSPSTS KAQDAGLAAK PQSTGISEVA AVSTTIWPSS AVYQSGSSLW AEEKATESPP TIALSTQAST TSSPKQNVGS EGQPPWVQEQ DSTPEKSPGP EETNPVHTDI FQDRGPGSTV HPSVAPTSSEKTPSPELVASGSQAPKVEEP IHATADPQKL SVFITPVPDS QAATRRQ
• Sequence Length: 393

• Applicable Applications for CX3CL1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Trp81~Gln337 (Accession # O55145) with N-terminal His-Tag
• Subcellular Location: Cell membrane; Single-pass type I membrane protein, secreted.
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 5.6
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 19745170
• NCBI GeneID: 89808
• NCBI Accession #: NP_604450.1
• NCBI GenBank Nucleotide #: NM_134455.1
• UniProt Accession #: O55145
• Molecular Weight: 28.4kDa
• NCBI Official Full Name: fractalkine
• NCBI Official Synonym Full Names: chemokine (C-X3-C motif) ligand 1
• NCBI Official Symbol: Cx3cl1
• NCBI Official Synonym Symbols: Cx3c; Scyd1
• NCBI Protein Information: fractalkine; neurotactin; C-X3-C motif chemokine 1; small-inducible cytokine D1; CX3C membrane-anchored chemokine; small inducible cytokine subfamily D, 1
• UniProt Protein Name: Fractalkine
• Protein Family: Fractalkine
• UniProt Gene Name: Cx3cl1
• UniProt Synonym Gene Names: Acc1; Fkn; Scyd1
• UniProt Entry Name: X3CL1_RAT

NCBI Description

chemokine that binds its receptor CX3CR1; induces chemotaxis and increased intracellular calcium levels in microglia [RGD, Feb 2006]

Uniprot Description

Function: The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1.

Subunit structure: Monomer

By similarity.

Subcellular location: Cell membrane; Single-pass type I membrane protein

By similarity. Processed fractalkine: Secreted

By similarity.

Tissue specificity: Highest levels in brain (neurons). Significant levels in kidney, heart, lung and adrenal gland.

Post-translational modification: A soluble short 95 kDa form may be released by proteolytic cleavage from the long membrane-anchored form.

Sequence similarities: Belongs to the intercrine delta family.