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Western Blot (WB) (Western Blot; Sample: Recombinant SLPI, Mouse.)

Rabbit anti-Mouse Secretory Leukocyte Peptidase Inhibitor (SLPI) Polyclonal Antibody | anti-SLPI antibody

Polyclonal Antibody to Secretory Leukocyte Peptidase Inhibitor (SLPI)

Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Secretory Leukocyte Peptidase Inhibitor (SLPI); Polyclonal Antibody; Polyclonal Antibody to Secretory Leukocyte Peptidase Inhibitor (SLPI); anti-SLPI antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Applicable Applications for anti-SLPI antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Western blotting: 0.5-2 ug/mL;1:500-2000
Immunohistochemistry: 5-20ug/mL;1:50-200
Immunocytochemistry: 5-20 ug/mL;1:50-200

Optimal working dilutions must be determined by end user.
Organism Species
Mus musculus (Mouse)
Source
Polyclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant SLPI (Pro20~Met131) expressed in E.coli
Cross Reactivity
Mouse
Preparation and Storage
Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot; Sample: Recombinant SLPI, Mouse.)

Western Blot (WB) (Western Blot; Sample: Recombinant SLPI, Mouse.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Esophagus Tissue; DAB staining on IHC-P; Samples: Mouse Testis Tissue; Primary Ab: 20ug/ml Rabbit Anti-MouseSLPI Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Esophagus Tissue; DAB staining on IHC-P; Samples: Mouse Testis Tissue; Primary Ab: 20ug/ml Rabbit Anti-MouseSLPI Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Testis Tissue; Primary Ab: 20ug/ml Rabbit Anti-Mouse SLPI Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Testis Tissue; Primary Ab: 20ug/ml Rabbit Anti-Mouse SLPI Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
antileukoproteinase
NCBI Official Synonym Full Names
secretory leukocyte peptidase inhibitor
NCBI Official Symbol
Slpi
NCBI Protein Information
antileukoproteinase
UniProt Protein Name
Antileukoproteinase
Protein Family
UniProt Gene Name
Slpi
UniProt Synonym Gene Names
ALP

Uniprot Description

SLPI: Acid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G. May prevent elastase-mediated damage to oral and possibly other mucosal tissues.

Protein type: Inhibitor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2|2 H3

Cellular Component: extracellular matrix; extracellular region; extracellular space; Golgi apparatus

Molecular Function: DNA binding; endopeptidase inhibitor activity; enzyme binding; mRNA binding; peptidase inhibitor activity; serine-type endopeptidase inhibitor activity

Biological Process: antibacterial humoral response; defense response to bacterium; immune response; immune system process; innate immune response; negative regulation of peptidase activity; negative regulation of protein binding; negative regulation of viral genome replication; response to lipopolysaccharide

Research Articles on SLPI

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Product Notes

The SLPI slpi (Catalog #AAA2003421) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Secretory Leukocyte Peptidase Inhibitor (SLPI) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Secretory Leukocyte Peptidase Inhibitor (SLPI) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, Western Blot (WB), Immunoprecipitation (IP). Western blotting: 0.5-2 ug/mL;1:500-2000 Immunohistochemistry: 5-20ug/mL;1:50-200 Immunocytochemistry: 5-20 ug/mL;1:50-200 Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the SLPI slpi for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Secretory Leukocyte Peptidase Inhibitor (SLPI), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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