Western Blot (WB)
(Western Blot: Sample: Rat Heart lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse PPARg Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Mouse Peroxisome Proliferator Activated Receptor Gamma (PPARg) Polyclonal Antibody | anti-PPARg antibody
Polyclonal Antibody to Peroxisome Proliferator Activated Receptor Gamma (PPARg)
MGHHHHHHSGSEF- FH YGVHACEGCK GFFRRTIRLK LIYDRCDLNC RIHKKSRNKC QYCRFQKCLA VGMSHNAIRF GRMPQAEKEK LLAEISSDID QLNPESADLR ALAKHLYDSY IKSFPLTKAK ARAILTGKTT DKS
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Rat Heart lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse PPARg Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Rat Heart lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse PPARg Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded esophagus tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded esophagus tissue))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a nuclear receptor protein belonging to the peroxisome proliferator-activated receptor (Ppar) family. The encoded protein is a ligand-activated transcription factor that is involved in the regulation of adipocyte differentiation and glucose homeostasis. The encoded protein forms a heterodimer with retinoid X receptors and binds to DNA motifs termed "peroxisome proliferator response elements" to either activate or inhibit gene expression. Mice lacking the encoded protein die at an embryonic stage due to severe defects in placental vascularization. When the embryos lacking this gene are supplemented with healthy placentas, the mutants survive to term, but succumb to lipodystrophy and multiple hemorrhages. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]
Uniprot Description
PPAR-gamma: a transcription factor, member of the nuclear hormone receptor superfamily. Receptor for hypolipidemic drugs and fatty acids. Preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage. Regulator of adipogenesis and lipid metabolism, modulates insulin sensitivity, cell proliferation and inflammation. Phosphorylated and inhibited by MAP kinase. Heterodimerizes with the retinoid X receptor. Interacts with NCOA6 coactivator, leading to a strong increase in transcription of target genes. Two splice-variant isoforms have been described.
Protein type: DNA-binding; Nuclear receptor
Chromosomal Location of Human Ortholog: 6 E3|6 53.41 cM
Cellular Component: cytoplasm; cytosol; intracellular membrane-bound organelle; nucleus; perinuclear region of cytoplasm; protein complex; RNA polymerase II transcription factor complex
Molecular Function: activating transcription factor binding; alpha-actinin binding; arachidonic acid binding; chromatin binding; DBD domain binding; DNA binding; DNA binding transcription factor activity; double-stranded DNA binding; drug binding; enzyme binding; estrogen receptor binding; identical protein binding; LBD domain binding; ligand-dependent nuclear receptor activity; ligand-dependent nuclear receptor transcription coactivator activity; metal ion binding; peptide binding; protein binding; protein C-terminus binding; protein heterodimerization activity; protein phosphatase binding; protein self-association; retinoid X receptor binding; sequence-specific DNA binding; steroid hormone receptor activity; transcription factor binding; transcription regulatory region DNA binding; WW domain binding; zinc ion binding
Biological Process: activation of cysteine-type endopeptidase activity involved in apoptotic process; brown fat cell differentiation; cell fate commitment; cell maturation; cellular response to insulin stimulus; cellular response to lithium ion; cellular response to organic cyclic compound; diet induced thermogenesis; epithelial cell differentiation; fat cell differentiation; fatty acid oxidation; glucose homeostasis; inflammatory response; lipoprotein transport; long-chain fatty acid transport; low-density lipoprotein receptor biosynthetic process; macrophage derived foam cell differentiation; monocyte differentiation; negative regulation of acute inflammatory response; negative regulation of angiogenesis; negative regulation of blood vessel endothelial cell migration; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of cellular response to insulin stimulus; negative regulation of cholesterol storage; negative regulation of collagen biosynthetic process; negative regulation of cytokine production; negative regulation of macrophage derived foam cell differentiation; negative regulation of pancreatic stellate cell proliferation; negative regulation of peptide hormone secretion; negative regulation of receptor biosynthetic process; negative regulation of sequestering of triglyceride; negative regulation of smooth muscle cell proliferation; negative regulation of telomerase activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; placenta development; positive regulation of apoptosis; positive regulation of DNA binding; positive regulation of fat cell differentiation; positive regulation of fatty acid oxidation; positive regulation of oligodendrocyte differentiation; positive regulation of phagocytosis, engulfment; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; regulation of blood pressure; regulation of circadian rhythm; regulation of fat cell differentiation; regulation of gene expression; regulation of lipid metabolic process; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; response to dietary excess; response to food; response to light stimulus; response to lipid; response to low density lipoprotein stimulus; response to retinoic acid; rhythmic process; signal transduction; steroid hormone mediated signaling; transcription, DNA-dependent; white fat cell differentiation
Research Articles on PPARg
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Product Notes
The PPARg pparg (Catalog #AAA2003418) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Peroxisome Proliferator Activated Receptor Gamma (PPARg) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Peroxisome Proliferator Activated Receptor Gamma (PPARg) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the PPARg pparg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- FH YGVHACEGCK GFFRRTIRLK LIYDRCDLNC RIHKKSRNKC QYCRFQKCLA VGMSHNAIRF GRMPQAEKEK LLAEISSDID QLNPESADLR ALAKHLYDSY IKSFPLTKAK ARAILTGKTT DKS. It is sometimes possible for the material contained within the vial of "Peroxisome Proliferator Activated Receptor Gamma (PPARg), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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