Mouse anti-Human SSR4 Monoclonal Antibody | anti-SSR4 antibody

SSR4 antibody

Cat. Num. AAA200275

• Gene Names: SSR4; CDG1Y; TRAPD
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: By protein-A affinity chromatography
• Synonyms: SSR4; Monoclonal Antibody; SSR4 antibody; Translocon-associated protein subunit delta; TRAPD; anti-SSR4 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: AT26G5
• Purity/Purification: By protein-A affinity chromatography
• Form/Format: Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 173

• Applicable Applications for anti-SSR4 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Recommended starting dilution is 1:1000.
• Antigen Species: Human
• Immunogen: Recombinant human SSR4 (24-144aa) purified from E. coli
• Clone Note: Anti-human SSR4 mAb, clone AT26G5, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human SSR4 protein.
• Preparation and Storage: Can be stored at 4 degree C.; For long term storage, aliquot and store at -20 degree C.; Avoid repeated freezing and thawing cycles.

Western Blot (WB)

(The lysate of MCF7 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SSR4 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)

Related Product Information for anti-SSR4 antibody

SSR4, also called TRAPD, is assumed to be involved in protein secretion. It is located in the Xq28 region, arranged in a compact head-to-head manner with the IDH3G gene. These two genes are driven by a bidirectional promoter located between them, and encode proteins involved in unrelated biochemical pathways located in different compartments of the cell. The nontranscribed intergenic region represents only 133 bp and is embedded in a CpG island. The CpG island functions as a bidirectional promoter to initiate the transcription of both functionally unrelated genes with distinct expression patterns. SSR4 consists of six exons and is approximately 70 kb telomeric to the ALD gene. Although alternative splicing of exon 5 has not been detected in human SSR4, transcript variants missing the region homologous to human exon 5 have been detected in both Xenopus laevis and Mus musculus.

Product Categories/Family for anti-SSR4 antibody

Signal Transduction

References

Brenner V., et al. (1997) Genomics 44 (1): 8-14; Holthuis JC., et al. (1996) J Biol Chem 312 (Pt 1) (Pt 1): 205-13; Entrez Gene: SSR4 signal sequence receptor, delta

NCBI and Uniprot Product Information

• NCBI GI #: 5454090
• NCBI GeneID: 6748
• NCBI Accession #: NP_006271
• NCBI GenBank Nucleotide #: NM_006280.2
• UniProt Accession #: P51571; Q53XY1; A8K378
• Molecular Weight: 18,999 Da
• NCBI Official Full Name: translocon-associated protein subunit delta isoform 2
• NCBI Official Synonym Full Names: signal sequence receptor subunit 4
• NCBI Official Symbol: SSR4
• NCBI Official Synonym Symbols: CDG1Y; TRAPD
• NCBI Protein Information: translocon-associated protein subunit delta
• UniProt Protein Name: Translocon-associated protein subunit delta
• Protein Family: SWI/SNF and RSC complexes
• UniProt Gene Name: SSR4
• UniProt Synonym Gene Names: TRAPD; TRAP-delta; SSR-delta

NCBI Description

This gene encodes the delta subunit of the translocon-associated protein complex which is involved in translocating proteins across the endoplasmic reticulum membrane. The encoded protein is located in the Xq28 region and is arranged in a compact head-to-head manner with the isocitrate dehydrogenase 3 (NAD+) gamma gene and both genes are driven by a CpG-embedded bidirectional promoter. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Mar 2011]

Uniprot Description

TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins.