Western Blot (WB)
(Western Blot: Sample: Mouse Spleen lysate; Primary Ab: 2ug/mL Rabbit Anti-Mouse MEC Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047
))
Rabbit Mucosae Associated Epithelia Chemokine (MEC) Polyclonal Antibody | anti-MEC antibody
Polyclonal Antibody to Mucosae Associated Epithelia Chemokine (MEC)
Gene Names
Ccl28; MEC; CCK1; Scya28
Reactivity
Mus musculus (Mouse)
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Mucosae Associated Epithelia Chemokine (MEC); Polyclonal Antibody; Polyclonal Antibody to Mucosae Associated Epithelia Chemokine (MEC); anti-MEC antibody
Host
Rabbit
Reactivity
Mus musculus (Mouse)
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-MEC antibody
Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).
Application Notes
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Source
Polyclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant MEC (Phe17~Arg130 (Accession # Q9JIL2)) expressed in E.coli (MBS2012326).
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Mouse Spleen lysate; Primary Ab: 2ug/mL Rabbit Anti-Mouse MEC Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047 ))
Western Blot (WB)
(Western Blot: Sample: Mouse Spleen lysate; Primary Ab: 2ug/mL Rabbit Anti-Mouse MEC Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047
))
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.MEC, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.MEC, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
C-C motif chemokine 28
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 28
NCBI Official Symbol
Ccl28
NCBI Official Synonym Symbols
MEC; CCK1; Scya28
NCBI Protein Information
C-C motif chemokine 28
UniProt Protein Name
C-C motif chemokine 28
Protein Family
UniProt Gene Name
Ccl28
UniProt Synonym Gene Names
Scya28
Uniprot Description
CCL28: Chemotactic activity for resting CD4, CD8 T-cells and eosinophils. Binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner. Belongs to the intercrine beta (chemokine CC) family.
Protein type: Chemokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 13|13 D2.3
Cellular Component: extracellular exosome; extracellular region; extracellular space
Molecular Function: chemokine activity; cytokine activity
Biological Process: chemotaxis; elevation of cytosolic calcium ion concentration; immune response; positive regulation of cell-matrix adhesion
Research Articles on MEC
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Product Notes
The MEC ccl28 (Catalog #AAA2002295) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Mucosae Associated Epithelia Chemokine (MEC) reacts with Mus musculus (Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's Mucosae Associated Epithelia Chemokine (MEC) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the MEC ccl28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mucosae Associated Epithelia Chemokine (MEC), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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