Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Brain Tissue; Lane2: Mouse Spleen Tissue.)
Rabbit anti-Mouse Nucleolin (NCL) Polyclonal Antibody | anti-NCL antibody
Polyclonal Antibody to Nucleolin (NCL)
MGHHHHHHSGSEF- YVDFESAE DLEKALELTG LKVFGNEIKL EKPKGRDSKK VRAARTLLAK NLSFNITEDE LKEVFEDAME IRLVSQDGKS KGIAYIEFKS EADAEKNLEE KQGAEIDGRS VSLYYTGEKG QRQERTGKTS TWSGESKTLV LSNLSYSATK ETLEEVFEKA TFIKVPQNPH GKPKGYAFIE FASFEDAKEA LNSCNKMEIE GRTIRLELQG SNSRSQPS
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Brain Tissue; Lane2: Mouse Spleen Tissue.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Brain Tissue; Lane2: Mouse Spleen Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant NCL, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant NCL, Mouse.)
NCBI and Uniprot Product Information
Uniprot Description
NCL: the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and preribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-RNA transcription and ribosome assembly.
Protein type: DNA-binding; Helicase; Nuclear receptor co-regulator; Nucleolus; RNA-binding
Chromosomal Location of Human Ortholog: 1 C5|1 43.94 cM
Cellular Component: cell cortex; cell surface; cytoplasm; cytoplasmic ribonucleoprotein granule; dense fibrillar component; extracellular exosome; fibrillar center; intracellular ribonucleoprotein complex; membrane; nucleolus; nucleoplasm; nucleus
Molecular Function: calcium ion binding; DNA binding; DNA topoisomerase binding; ErbB-4 class receptor binding; histone binding; identical protein binding; laminin binding; mRNA 5'-UTR binding; nucleic acid binding; protein binding; protein C-terminus binding; receptor binding; RNA binding; rRNA primary transcript binding; sequence-specific DNA binding; single-stranded DNA binding; telomeric DNA binding
Biological Process: angiogenesis; cellular response to epidermal growth factor stimulus; endocytosis; negative regulation of apoptosis; negative regulation of translation; positive regulation of interleukin-6 secretion; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tumor necrosis factor production; regulation of rRNA processing
Research Articles on NCL
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Product Notes
The NCL ncl (Catalog #AAA2002282) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Nucleolin (NCL) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Nucleolin (NCL) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the NCL ncl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- YVDFESAE DLEKALELTG LKVFGNEIKL EKPKGRDSKK VRAARTLLAK NLSFNITEDE LKEVFEDAME IRLVSQDGKS KGIAYIEFKS EADAEKNLEE KQGAEIDGRS VSLYYTGEKG QRQERTGKTS TWSGESKTLV LSNLSYSATK ETLEEVFEKA TFIKVPQNPH GKPKGYAFIE FASFEDAKEA LNSCNKMEIE GRTIRLELQG SNSRSQPS. It is sometimes possible for the material contained within the vial of "Nucleolin (NCL), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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