Western Blot (WB)
(Western Blot: Sample: Lane1: Human Liver Tissue; Lane2: Human Lung Tissue; Lane3: Mouse Brain Tissue.)
Rabbit anti-Human Nucleolin (NCL) Polyclonal Antibody | anti-NCL antibody
Polyclonal Antibody to Nucleolin (NCL)
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- KER DARTLLAKNL PYKVTQDELK EVFEDAAEIR LVSKDGKSKG IAYIEFKTEA DAEKTFEEKQ GTEIDGRSIS LYYTGEKGQN QDYRGGKNST WSGESKTLVL SNLSYSATEE TLQEVFEKAT FIKVPQNQNG KSKGYAFIEF ASFEDAKEAL NSCNKREIEG RAIRLELQGP RGSPNARSQ
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Liver Tissue; Lane2: Human Lung Tissue; Lane3: Mouse Brain Tissue.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Liver Tissue; Lane2: Human Lung Tissue; Lane3: Mouse Brain Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant NCL, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant NCL, Human.)
NCBI and Uniprot Product Information
NCBI Description
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008]
Uniprot Description
NCL: the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and preribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-RNA transcription and ribosome assembly.
Protein type: DNA-binding; Helicase; Nuclear receptor co-regulator; Nucleolus; RNA-binding
Chromosomal Location of Human Ortholog: 2q37.1
Cellular Component: cell cortex; cytoplasmic ribonucleoprotein granule; extracellular exosome; intracellular ribonucleoprotein complex; membrane; nucleolus; nucleoplasm; nucleus
Molecular Function: DNA topoisomerase binding; identical protein binding; mRNA 5'-UTR binding; protein binding; protein C-terminus binding; RNA binding; telomeric DNA binding
Biological Process: angiogenesis; cellular response to epidermal growth factor stimulus; negative regulation of translation; positive regulation of transcription from RNA polymerase II promoter
Research Articles on NCL
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Product Notes
The NCL ncl (Catalog #AAA2005982) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Nucleolin (NCL) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Nucleolin (NCL) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the NCL ncl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- KER DARTLLAKNL PYKVTQDELK EVFEDAAEIR LVSKDGKSKG IAYIEFKTEA DAEKTFEEKQ GTEIDGRSIS LYYTGEKGQN QDYRGGKNST WSGESKTLVL SNLSYSATEE TLQEVFEKAT FIKVPQNQNG KSKGYAFIEF ASFEDAKEAL NSCNKREIEG RAIRLELQGP RGSPNARSQ. It is sometimes possible for the material contained within the vial of "Nucleolin (NCL), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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