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Western Blot (WB) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human cellubrevin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: Cellubrevin transfected 293T cell lysate The mouse brain tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human cellubrevin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)

Mouse anti-Human, Mouse Cellubrevin Monoclonal Antibody | anti-VAMP3 antibody

Cellubrevin antibody

Gene Names
VAMP3; CEB
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay
Purity
By protein-G affinity chromatography
Synonyms
Cellubrevin; Monoclonal Antibody; Cellubrevin antibody; Vesicle-associated membrane protein 3; VAMP 3; CEB; Synaptobrevin-3; Synaptobrevin 3; VAMP3; Vesicle associated membrane protein 3.; anti-VAMP3 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT4G9
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
100
Applicable Applications for anti-VAMP3 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Antigen Species
Human
Immunogen
Recombinant human cellubrevin (1-77aa) purified from E. coli
Clone Note
Anti-human cellubrevin mAb, clone AT4G9, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human cellubrevin protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.

Western Blot (WB)

(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human cellubrevin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: Cellubrevin transfected 293T cell lysate The mouse brain tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human cellubrevin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)

Western Blot (WB) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human cellubrevin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: Cellubrevin transfected 293T cell lysate The mouse brain tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human cellubrevin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Related Product Information for anti-VAMP3 antibody
Cellubrevin, also known as VAMP3, is a member of the Vamp/synaptobrevin family. Vamps, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. Cellubrevin is a vesicular soluble N-ethylmaleimide sensitive factor attachment protein receptor (v-SNARE) homologous to the neuronal synaptobrevins 1/2 and is also a substrate of tetanus neurotoxin.
Product Categories/Family for anti-VAMP3 antibody
References
Proux-G V., et al. (2005) Proc Natl Acad Sci USA. 102 (18):6362-6367; Randhawa Vk., et al. (2000) Mol Cell Biol. 11 (7):2403-2417; Olson AL., et al. (1997) Mol Cell Biol. 17 (5):2425-2435

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
11,309 Da
NCBI Official Full Name
vesicle-associated membrane protein 3
NCBI Official Synonym Full Names
vesicle associated membrane protein 3
NCBI Official Symbol
VAMP3
NCBI Official Synonym Symbols
CEB
NCBI Protein Information
vesicle-associated membrane protein 3
UniProt Protein Name
Vesicle-associated membrane protein 3
UniProt Gene Name
VAMP3
UniProt Synonym Gene Names
SYB3; VAMP-3; CEB

NCBI Description

Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Because of its high homology to other known VAMPs, its broad tissue distribution, and its subcellular localization, the protein encoded by this gene was shown to be the human equivalent of the rodent cellubrevin. In platelets the protein resides on a compartment that is not mobilized to the plasma membrane on calcium or thrombin stimulation. [provided by RefSeq, Jul 2008]

Uniprot Description

SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network.

Research Articles on VAMP3

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Product Notes

The VAMP3 vamp3 (Catalog #AAA200122) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Cellubrevin antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Cellubrevin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the VAMP3 vamp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cellubrevin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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