Chemokine Like Factor Superfamily 8 (CKLFSF8) CLIA Kit | CKLFSF8 clia kit

CLIA Kit for Chemokine Like Factor Superfamily 8 (CKLFSF8)

Cat. Num. AAA2001037

• Gene Names: CMTM8; CKLFSF8; CKLFSF8-V2
• Synonyms: Chemokine Like Factor Superfamily 8 (CKLFSF8); CLIA Kit for Chemokine Like Factor Superfamily 8 (CKLFSF8); CKLFSF8 clia kit

• Specificity: This assay has high sensitivity and excellent specificity for detection of Chemokine Like Factor Superfamily 8 (CKLFSF8); No significant cross-reactivity or interference between Chemokine Like Factor Superfamily 8 (CKLFSF8) and analogues was observed.
• Sequence Length: 173

• Assay Type: Sandwich
• Samples: Tissue homogenates and other biological fluids
• Detection Range: 2.743-2000pg/mL The standard curve concentrations used for the ELISA's were 2000pg/mL, 666.67pg/mL, 222.22pg/mL, 74.07pg/mL, 24.691pg/mL, 8.23pg/mL, 2.743pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 11.9pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine Like Factor Superfamily 8 (CKLFSF8) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine Like Factor Superfamily 8 (CKLFSF8) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Organism Species: Rattus norvegicus (Rat)
• Assay Length: 4.5 hours
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CKLFSF8 clia kit

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Chemokine Like Factor Superfamily 8 (CKLFSF8) level in the sample or standard.

NCBI and Uniprot Product Information

• NCBI GI #: 32130536
• NCBI GeneID: 152189
• NCBI Accession #: NP_849199.2
• NCBI GenBank Nucleotide #: NM_178868.3
• UniProt Accession #: Q8IZV2; Q8IW01; A5D6I7
• Molecular Weight: 19,572 Da
• NCBI Official Full Name: CKLF-like MARVEL transmembrane domain-containing protein 8
• NCBI Official Synonym Full Names: CKLF-like MARVEL transmembrane domain containing 8
• NCBI Official Symbol: CMTM8
• NCBI Official Synonym Symbols: CKLFSF8; CKLFSF8-V2
• NCBI Protein Information: CKLF-like MARVEL transmembrane domain-containing protein 8; chemokine-like factor superfamily 8; chemokine-like factor super family 8; chemokine-like factor superfamily member 8
• UniProt Protein Name: CKLF-like MARVEL transmembrane domain-containing protein 8
• UniProt Gene Name: CMTM8
• UniProt Synonym Gene Names: CKLFSF8
• UniProt Entry Name: CKLF8_HUMAN

NCBI Description

This gene belongs to the chemokine-like factor gene superfamily, a novel family that is similar to the chemokine and the transmembrane 4 superfamilies. This gene is one of several chemokine-like factor genes located in a cluster on chromosome 3. This gene is widely expressed in many tissues, but the exact function of the encoded protein is unknown. [provided by RefSeq, Jul 2008]

Uniprot Description

CMTM8: a widely distributed protein with four transmembrane regions and a MARVEL domain. Associates with the epidermal growth factor receptor. Transfection into several cell lines suppresses EGF-induced cell proliferation and increases the rate of receptor endocytosis. The lack of EGFR-mediated signaling induces cells to undergo apoptosis via caspase-dependent and -independent pathways. Knockdown of CMTM8 by siRNA promotes cell proliferation and delayed EGFR endocytosis. Two splice-variant human isoforms have been reproted.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 3p22.3

Cellular Component: extracellular space; cytoplasm; integral to membrane; nucleus

Molecular Function: cytokine activity

Biological Process: chemotaxis