Western Blot (WB)
(The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPP1R14A antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : A549 cell lysate Lane 2. : NIH-3T3 cell lysate )
Mouse anti-Human PPP1R14A Monoclonal Antibody | anti-PPP1R14A antibody
PPP1R14A antibody
Gene Names
PPP1R14A; CPI17; CPI-17; PPP1INL
Reactivity
Human
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence
Purity
By protein-G affinity chromatography
Synonyms
PPP1R14A; Monoclonal Antibody; PPP1R14A antibody; Protein phosphatase 1 regulatory (inhibitor) subunit 14A; CPI17; CPI-17; PPP1INL; Protein phosphatase 1; regulatory (inhibitor) subunit 14A; regulatory (inhibitor) subunit 14A CPI 17; CPI 17 alpha; CPI 17alpha; PKC potentiated inhibitory protein of PP1; Protein phosphatase 1 regulatory subunit 14A; Regulatory subunit 14A; ; anti-PPP1R14A antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
4H10
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
147
Applicable Applications for anti-PPP1R14A antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
WB: 1:500 ~ 1,000.
Recommended starting dilution is 1:500.
Recommended starting dilution is 1:500.
Antigen Species
Human
Immunogen
Recombinant human PPP1R14A (1-147aa) purified from E. coli
Clone Note
Anti-human PPP1R14A mAb, clone 4H10, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PPP1R14A protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPP1R14A antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : A549 cell lysate Lane 2. : NIH-3T3 cell lysate )
Western Blot (WB)
(The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPP1R14A antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : A549 cell lysate Lane 2. : NIH-3T3 cell lysate )
Immunofluorescence (IF)
(ICC/IF analysis of PPP1R14A in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PPP1R14A antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Immunofluorescence (IF)
(ICC/IF analysis of PPP1R14A in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PPP1R14A antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Related Product Information for anti-PPP1R14A antibody
PPP1R14A (Protein phosphatase 1 regulatory subunit 14A) is a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphate. Myosin phosphatase can reverse MYL (myosin light chain) phosphorylation to induce a state of relaxation. However, during agonist-induced contraction at constant Ca2+ concurrent inhibition of myosin phosphatase leads to increases in MYL phosphorylation and tension. These calcium-independent increases in myosin phosphorylation and tension are termed calcium sensitization.
Product Categories/Family for anti-PPP1R14A antibody
References
Hamaguchi., et al. (2000) Biochem. Biophys. Res Commun. 274 (3):825-30; Eto M., et al. (1997) FEBS Lett. 410 (2-3):356-60; Lartey I., et al. (2007) Biol Reprod. 76 (6):971-82
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13,480 Da
NCBI Official Full Name
protein phosphatase 1 regulatory subunit 14A isoform 1
NCBI Official Synonym Full Names
protein phosphatase 1 regulatory inhibitor subunit 14A
NCBI Official Symbol
PPP1R14A
NCBI Official Synonym Symbols
CPI17; CPI-17; PPP1INL
NCBI Protein Information
protein phosphatase 1 regulatory subunit 14A
UniProt Protein Name
Protein phosphatase 1 regulatory subunit 14A
UniProt Gene Name
PPP1R14A
UniProt Synonym Gene Names
CPI17; PPP1INL; CPI-17
NCBI Description
The protein encoded by this gene belongs to the protein phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Sep 2011]
Uniprot Description
Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.
Research Articles on PPP1R14A
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Product Notes
The PPP1R14A ppp1r14a (Catalog #AAA200095) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PPP1R14A antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PPP1R14A can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:500 ~ 1,000. Recommended starting dilution is 1:500. Researchers should empirically determine the suitability of the PPP1R14A ppp1r14a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPP1R14A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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