PPP1R14A recombinant protein

PPP1R14A, 1-147aa, Human, His tag, E Coli

Cat. Num. AAA203162

• Gene Names: PPP1R14A; CPI17; CPI-17; PPP1INL
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: PPP1R14A; 1-147aa; Human; His tag; E Coli; Protein phosphatase 1 regulatory (inhibitor) subunit 14A; CPI17; CPI-17; PPP1INL; Protein phosphatase 1; regulatory (inhibitor) subunit 14A; regulatory (inhibitor) subunit 14A CPI 17; CPI 17 alpha; CPI 17alpha; PKC potentiated inhibitory protein of PP1; Protein phosphatase 1 regulatory subunit 14A; Regulatory subunit 14A; ; PPP1R14A recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid in 20mM Tris pH 8.0, 2mM EDTA, 1mM DTT, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MAAQRLGKRV LSKLQSPSRA RGPGGSPGGL QKRHARVTVK YDRRELQRRL DVEKWIDGRL EELYRGMEAD MPDEINIDEL LELESEEERS RKIQGLLKSC GKPVEDFIQE LLAKLQGLHR QPGLRQPSPS HDGSLSPLQD RARTAHP
• Sequence Length: 147

• Applicable Applications for PPP1R14A recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PPP1R14A recombinant protein

PPP1R14A (Protein phosphatase 1 regulatory subunit 14A) is a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphate. Myosin phosphatase can reverse MYL (myosin light chain) phosphorylation to induce a state of relaxation. However, during agonist-induced contraction at constant Ca2+ concurrent inhibition of myosin phosphatase leads to increases in MYL phosphorylation and tension. These calcium-independent increases in myosin phosphorylation and tension are termed calcium sensitization. Recombinant His tagged PPP1R14A was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for PPP1R14A recombinant protein

Signal Transduction

References

Hamaguchi., et al. (2000). Biochem. Biophys. Res Commun. 274 (3):825-30.; Eto M., et al. (1997). FEBS Lett. 410 (2-3):356-60.; Lartey I., et al. (2007). Biol Reprod. 76 (6):971-82.;

NCBI and Uniprot Product Information

• NCBI GI #: 15147250
• NCBI GeneID: 94274
• NCBI Accession #: NP_150281
• NCBI GenBank Nucleotide #: NM_033256.2
• UniProt Accession #: Q96A00; Q7Z4X7; Q96S54
• Molecular Weight: 18 kDa (167 aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: protein phosphatase 1 regulatory subunit 14A isoform 1
• NCBI Official Synonym Full Names: protein phosphatase 1 regulatory inhibitor subunit 14A
• NCBI Official Symbol: PPP1R14A
• NCBI Official Synonym Symbols: CPI17; CPI-17; PPP1INL
• NCBI Protein Information: protein phosphatase 1 regulatory subunit 14A
• UniProt Protein Name: Protein phosphatase 1 regulatory subunit 14A
• UniProt Gene Name: PPP1R14A
• UniProt Synonym Gene Names: CPI17; PPP1INL; CPI-17

NCBI Description

The protein encoded by this gene belongs to the protein phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.