Rat Fibroblast Growth Factor 13 (FGF13) CLIA Kit | FGF13 clia kit

Rat Fibroblast Growth Factor 13 (FGF13) CLIA Kit

Cat. Num. AAA2000408

• Gene Names: Fgf13; Fhf2
• Reactivity: Rat
• Synonyms: Fibroblast Growth Factor 13 (FGF13); Rat Fibroblast Growth Factor 13 (FGF13) CLIA Kit; FHF2; Fibroblast Growth Factor Homologous Factor 2; FGF13 clia kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 13 (FGF13).; No significant cross-reactivity or interference between Fibroblast Growth Factor 13 (FGF13) and analogues was observed.
• Sequence Length: 245

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 1.37-1,000pg/mL
• Sensitivity: < 0.59pg/mL
• Application: Chemiluminescent immunoassay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 13 (FGF13) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 13 (FGF13) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGF13 clia kit

The microplate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 13 (FGF13). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 13 (FGF13). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Fibroblast Growth Factor 13 (FGF13) level in the sample or standard.;

Product Categories/Family for FGF13 clia kit

Cytokine

NCBI and Uniprot Product Information

• NCBI GI #: 31542809
• NCBI GeneID: 14168
• NCBI Accession #: NP_034330.2
• NCBI GenBank Nucleotide #: NM_010200.3
• UniProt Accession #: P70377; O35338; Q3UR31; Q8VCY9; Q9JLA5; B1AU21
• Molecular Weight: 28,760 Da
• NCBI Official Full Name: fibroblast growth factor 13 isoform 1
• NCBI Official Synonym Full Names: fibroblast growth factor 13
• NCBI Official Symbol: Fgf13
• NCBI Official Synonym Symbols: Fhf2
• NCBI Protein Information: fibroblast growth factor 13
• UniProt Protein Name: Fibroblast growth factor 13
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: Fgf13
• UniProt Synonym Gene Names: Fhf2; FGF-13; FHF-2
• UniProt Entry Name: FGF13_MOUSE

Uniprot Description

FGF13: Microtubule-binding protein which directly binds tubulin and is involved in both polymerization and stabilization of microtubules. Through its action on microtubules, may participate to the refinement of axons by negatively regulating axonal and leading processes branching. Plays a crucial role in neuron polarization and migration in the cerebral cortex and the hippocampus. Belongs to the heparin-binding growth factors family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Nucleolus

Cellular Component: microtubule; neuron projection; growth cone; cell projection; axon; dendrite; cytoplasm; extracellular region; plasma membrane; cytosol; nucleus; filopodium

Molecular Function: growth factor activity; sodium channel regulator activity; microtubule binding; beta-tubulin binding; protein kinase activator activity; receptor binding

Biological Process: cell proliferation; nervous system development; cerebral cortex cell migration; negative regulation of microtubule depolymerization; apoptosis; establishment of neuroblast polarity; positive regulation of protein kinase activity; hippocampus development; MAPKKK cascade; neuron migration; learning; negative regulation of collateral sprouting; memory; microtubule polymerization