Human Delta Like Protein 1 (dLL1) ELISA Kit | dLL1 elisa kit

Human Delta Like Protein 1 (dLL1) ELISA Kit

Cat. Num. AAA2000175

• Gene Names: DLL1; DL1; Delta; DELTA1
• Reactivity: Human
• Synonyms: Delta Like Protein 1 (dLL1); Human Delta Like Protein 1 (dLL1) ELISA Kit; Delta1; Drosophila Delta homolog 1; dLL1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Delta Like Protein 1 (dLL1).; No significant cross-reactivity or interference between Delta Like Protein 1 (dLL1) and analogues was observed.
• Sequence Length: 723

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.056ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Delta Like Protein 1 (dLL1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Delta Like Protein 1 (dLL1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for dLL1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Delta Like Protein 1 (dLL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Delta Like Protein 1 (dLL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Delta Like Protein 1 (dLL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Delta Like Protein 1 (dLL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for dLL1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 110735443
• NCBI GeneID: 28514
• NCBI Accession #: NP_005609.3
• NCBI GenBank Nucleotide #: NM_005618.3
• UniProt Accession #: O00548; Q9NU41; Q9UJV2; B2RAK7; B5M0B3
• Molecular Weight: 27,355 Da
• NCBI Official Full Name: delta-like protein 1
• NCBI Official Synonym Full Names: delta-like 1 (Drosophila)
• NCBI Official Symbol: DLL1
• NCBI Official Synonym Symbols: DL1; Delta; DELTA1
• NCBI Protein Information: delta-like protein 1
• UniProt Protein Name: Delta-like protein 1
• Protein Family: Delta-like protein
• UniProt Gene Name: DLL1
• UniProt Synonym Gene Names: Delta1; H-Delta-1
• UniProt Entry Name: DLL1_HUMAN

NCBI Description

DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis. It may play a role in cell-to-cell communication. [provided by RefSeq, Jul 2008]

Uniprot Description

DLL1: Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor. Interacts with Notch receptors. Expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell development/differentiation

Chromosomal Location of Human Ortholog: 6q27

Cellular Component: integral to plasma membrane; extracellular region; plasma membrane; cytoplasmic vesicle

Molecular Function: protein binding; calcium ion binding; Notch binding

Biological Process: regulation of cell adhesion; Notch signaling pathway; negative regulation of myeloid cell differentiation; negative regulation of interleukin-10 production; Notch receptor processing; cell fate determination; compartment specification; somite specification; cell-cell signaling; negative regulation of auditory receptor cell differentiation; positive regulation of transcription from RNA polymerase II promoter; hemopoiesis; heart looping; determination of left/right symmetry; cell differentiation; inner ear development; positive regulation of Notch signaling pathway