HspA5 (Hsp70 protein 5) Recombinant Protein | HspA5 recombinant protein

Recombinant Human HspA5 (Hsp70 protein 5)

Cat. Num. AAA197043

• Gene Names: HSPA5; BIP; MIF2; GRP78; FLJ26106
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: HspA5 (Hsp70 protein 5); Recombinant Human HspA5 (Hsp70 protein 5); HspA5 recombinant protein

• Host: E Coli
• Specificity: Human HspA5 (Hsp70 protein 5)
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: Sterile-filtered colorless solution in 20mM Tris-Hcl, pH 8.0 and 10% glycerol. Purified by proprietary chromatographic techniques.
• Sequence: EEDKKEDVG TVVGIDLGTT YSCVGVFKNG RVEIIANDQG NRITPSYVAF TPEGERLIGD AAKNQLTSNP ENTVFDAKRL IGRTWNDPSV QQDIKFLPFK VVEKKTKPYI QVDIGGGQTK TFAPEEISAM VLTKMKETAE AYLGKKVTHA VVTVPAYFND AQRQATKDAG TIAGLNVMRI INEPTAAAIA YGLDKREGEK NILVFDLGGG TFDVSLLTID NGVFEVVATN GDTHLGGEDF DQRVMEHFIK LYKKKTGKDV RKDNRAVQKL RREVEKAKRA LSSQHQARIE IESFYEGEDF SETLTRAKFE ELNMDLFRST MKPVQKVLED SDLKKSDIDE IVLVGGSTRI PKIQQLVKEF FNGKEPSRGI NPDEAVAYGA AVQAGVLSGD QDTGDLVLLD VCPLTLGIET VGGVMTKLIP RNTVVPTKKS QIFSTASDNQ PTVTIKVYEG ERPLTKDNHL LGTFDLTGIP PAPRGVPQIE VTFEIDVNGI LRVTAEDKGT GNKNKITITN DQNRLTPEEI ERMVNDAEKF AEEDKKLKER IDTRNELESY AYSLKNQIGD KEKLGGKLSS EDKETMEKAV EEKIEWLESH QDADIEDFKA KKKELEEIVQ PIISKLYGSA GPPPTGEEDT AELEHHHHHH

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store at or below -20 degree C for longer periods of time. Addition of a carrier protein (such as 0.1% HSA or BSA is recommended for long-term storage.

Related Product Information for HspA5 recombinant protein

HspA5, a member of the Hsp70 family, plays a role in folding and assembling of proteins in the endoplasmic reticulum by monitoring protein transport through the cell. HspA5 is a stress response protein that is induced by conditions that adversely affect endoplasmic reticulum function. HspA5 is essential to maintenance of cell homeostasis and prevention of apoptosis.

Recombinant human HspA5 produced in E. coli is a single, non-glycosylated polypeptide containing 640 amino acids (aa 2-650) with a molecular weight of 70kDa. It is fused to an 8-amino acid His-tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 16507237
• NCBI GeneID: 3309
• NCBI Accession #: NP_005338.1
• NCBI GenBank Nucleotide #: NM_005347.4
• UniProt Accession #: P11021; Q2EF78; Q9NPF1; Q9UK02; B0QZ61
• Molecular Weight: 72,333 Da
• NCBI Official Full Name: 78 kDa glucose-regulated protein
• NCBI Official Synonym Full Names: heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
• NCBI Official Symbol: HSPA5
• NCBI Official Synonym Symbols: BIP; MIF2; GRP78; FLJ26106
• NCBI Protein Information: 78 kDa glucose-regulated protein; OTTHUMP00000022124; immunoglobulin heavy chain-binding protein; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
• UniProt Protein Name: 78 kDa glucose-regulated protein
• UniProt Gene Name: HSPA5
• UniProt Synonym Gene Names: GRP78
• UniProt Entry Name: GRP78_HUMAN

NCBI Description

The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.

Uniprot Description

GRP78: a member of the HSP family of molecular chaperones required for endoplasmic reticulum integrity and stress-induced autophagy. Plays a central role in regulating the unfolded protein response (UPR), and is an obligatory component of autophagy in mammalian cells. May play an important role in cellular adaptation and oncogenic survival. One of the client proteins of GRP78 is protein double-stranded RNA-activated protein-like endoplasmic reticulum kinase (PERK). Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.

Protein type: Heat shock protein; Chaperone

Chromosomal Location of Human Ortholog: 9q33.3

Cellular Component: signalosome; endoplasmic reticulum membrane; cell surface; smooth endoplasmic reticulum; focal adhesion; mitochondrion; endoplasmic reticulum lumen; endoplasmic reticulum; ER-Golgi intermediate compartment; membrane; melanosome; plasma membrane; integral to endoplasmic reticulum membrane; midbody; nucleus

Molecular Function: protein domain specific binding; protein binding; enzyme binding; ubiquitin protein ligase binding; chaperone binding; unfolded protein binding; ATPase activity; ribosome binding; misfolded protein binding; calcium ion binding; glycoprotein binding; ATP binding

Biological Process: platelet activation; ER-associated protein catabolic process; cerebellum structural organization; unfolded protein response; cerebellar Purkinje cell layer development; cellular response to glucose starvation; platelet degranulation; cellular protein metabolic process; substantia nigra development; unfolded protein response, activation of signaling protein activity; positive regulation of protein ubiquitination; negative regulation of transforming growth factor beta receptor signaling pathway; blood coagulation; ER overload response; positive regulation of cell migration; negative regulation of apoptosis