alpha crystallin B Recombinant Protein | Cryab recombinant protein

Recombinant Mouse Alpha Crystallin B (CRYAB Mouse)

Cat. Num. AAA197038

• Gene Names: Cryab; Crya2; HspB5; Crya-2
• Purity: Greater than 95% as determined by SDS-PAGE and RP-HPLC.
• Synonyms: alpha crystallin B; Recombinant Mouse Alpha Crystallin B (CRYAB Mouse); Cryab recombinant protein

• Host: E Coli
• Specificity: Mouse alpha crystallin B
• Purity/Purification: Greater than 95% as determined by SDS-PAGE and RP-HPLC.
• Form/Format: Sterile-filtered colorless solution contains 20mM Tris buffer, pH 8.0 and 10% glycerol. Purified by proprietary chromatographic techniques.
• Sequence: MDIAIHHPWI RRPFFPFHSP SRLFDQFFGE HLLESDLFST ATSLSPFYLR PPSFLRAPSW IDTGLSEMRL EKDRFSVNLD VKHFSPEELK VKVLGDVIEV HGKHEERQDE HGFISREFHR KYRIPADVDP LTITSSLSSD GVLTVNGPRK QVSGPERTIP ITREEKPAVA AAPKK

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store at or below -20 degree C for longer periods of time. For long term storage, addition of a carrier protein (0.1% HSA or BSA) is recommended.

Related Product Information for Cryab recombinant protein

Alpha crystallins are composed of two gene products: alpha-A (acidic) and alpha-B (basic). Alpha crystallins are induced by heat shock and are members of the small heat shock protein family. They act as molecular chaperones. In addition, alpha crystallins have autokinase activity and play a role in intracellular architecture. Alpha crystallin B is expressed in many tissues and is associated with many neurological diseases.

Recombinant Mouse CRYAB produced in E. coli is a single, non-glycosylated polypeptide chain containing 175 amino acids with a molecular weight of 20kDa.

NCBI and Uniprot Product Information

• NCBI GI #: 6753530
• NCBI GeneID: 12955
• NCBI Accession #: NP_034094.1
• NCBI GenBank Nucleotide #: NM_009964.2
• UniProt Accession #: P23927; Q64325; Q52L78
• Molecular Weight: 20,069 Da
• NCBI Official Full Name: alpha-crystallin B chain
• NCBI Official Synonym Full Names: crystallin, alpha B
• NCBI Official Symbol: Cryab
• NCBI Official Synonym Symbols: Crya2; HspB5; Crya-2
• NCBI Protein Information: alpha-crystallin B chain; P23; alpha B-crystallin; alpha(B)-crystallin; crystallin, alpha 2
• UniProt Protein Name: Alpha-crystallin B chain
• Protein Family: Alpha-crystallin
• UniProt Gene Name: Cryab
• UniProt Synonym Gene Names: Crya2
• UniProt Entry Name: CRYAB_MOUSE

NCBI Description

This gene encodes a member of the small heat-shock protein (HSP20) family. The encoded protein is a molecular chaperone that protects proteins against thermal denaturation and other stresses. This protein is a component of the eye lens, regulates lens differentiation and functions as a refractive element in the lens. This protein is a negative regulator of inflammation, has anti-apoptotic properties and also plays a role in the formation of muscular tissue. Mice lacking this gene exhibit worse experimental autoimmune encephalomyelitis and inflammation of the central nervous system compared to the wild type. In mouse models, this gene has a critical role in alleviating the pathology of the neurodegenerative Alexander disease. Mutations in the human gene are associated with myofibrillar myopathy 2, fatal infantile hypertonic myofibrillar myopathy, multiple types of cataract and dilated cardiomyopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

CRYAB: a major structural protein of the eye lens. A member of the small heat shock protein (sHSP also known as the HSP20) family. Alpha-B is expressed in the lens as well as other tissues. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy.

Protein type: Heat shock protein; Chaperone

Cellular Component: microtubule cytoskeleton; I band; Golgi apparatus; cell surface; contractile fiber; mitochondrion; cytoplasm; actin filament bundle; plasma membrane; Z disc; nucleus; cytosol; myelin sheath

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; metal ion binding; microtubule binding; cytoskeletal protein binding; unfolded protein binding; structural constituent of eye lens

Biological Process: lens development in camera-type eye; muscle development; camera-type eye development; protein folding; microtubule polymerization or depolymerization; stress-activated MAPK cascade; negative regulation of caspase activity; tubulin folding; response to hydrogen peroxide; negative regulation of intracellular transport; response to hypoxia; negative regulation of cell growth; protein homooligomerization; negative regulation of apoptosis