Mouse Factor VIII Monoclonal Antibody | anti-F8 antibody

Factor VIII Monoclonal Antibodies

Cat. Num. AAA191415

• Gene Names: F8; AHF; F8B; F8C; HEMA; FVIII; DXS1253E
• Synonyms: Factor VIII; Monoclonal Antibody; Factor VIII Monoclonal Antibodies; Factor VIII-Library Pack; anti-F8 antibody

• Host: Mouse
• Clonality: Monoclonal
• Specificity: MBS190151, MBS190152, and MBS190153 recognize full-length human Factor VIII (epitopes not determined). 10104 recognizes an epitope at the N-terminal region of the 83kDa light chain of Factor VIII. These antibodies do not cross-react with von Willebrand factor.
• Form/Format: Protein G-purified antibody in PBS, pH 7.4.
• Sequence Length: 2351

• Application Notes: Dilute in PBS or medium that is identical to that used in the assay system.
• Immunogen: Purified human Factor VIII.
• Preparation and Storage: These antibodies are stable for at least one (1) year at-20 degree C to-70 degree C.; Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

Testing Data

(Detection of Factor VIII light chain (LCh) in wild-type (WT) and recombinant (R372H) Factor VIII after reaction with thrombin over time (Nogami et al. 2005 Blood 105: 4362-4368).)

Related Product Information for anti-F8 antibody

Factor VIII (FVIII) is an essential blood-clotting protein, also known as anti-hemophilic factor (AHF). In humans, factor VIII is encoded by the F8 gene. Defects in this gene result in hemophilia A, a recessive X-linked coagulation disorder. Factor VIII is produced in liver sinusoidal cells and endothelial cells outside of the liver throughout the body. This protein circulates in the bloodstream in an inactive form, bound to another molecule called von Willebrand factor, until an injury that damages blood vessels occurs. In response to injury, factor VIII is activated and separates from von Willebrand factor. The active protein (sometimes written as factor VIIIa) interacts with another coagulation factor called factor IX. This interaction sets off a chain of additional chemical reactions that form a blood clot.

NCBI and Uniprot Product Information

• NCBI GI #: 4503647
• NCBI GeneID: 2157
• NCBI Accession #: NP_000123
• NCBI GenBank Nucleotide #: NM_000132.3
• UniProt Accession #: P00451; Q14286; Q5HY69
• Molecular Weight: 24,641 Da
• NCBI Official Full Name: coagulation factor VIII isoform a preproprotein
• NCBI Official Synonym Full Names: coagulation factor VIII
• NCBI Official Symbol: F8
• NCBI Official Synonym Symbols: AHF; F8B; F8C; HEMA; FVIII; DXS1253E
• NCBI Protein Information: coagulation factor VIII
• UniProt Protein Name: Coagulation factor VIII
• Protein Family: Factor VIII intron 22 protein
• UniProt Gene Name: F8
• UniProt Synonym Gene Names: F8C; AHF

NCBI Description

This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder. [provided by RefSeq, Jul 2008]

Uniprot Description

Factor VIII, along with calcium and phospholipid, acts as a cofactor for F9/factor IXa when it converts F10/factor X to the activated form, factor Xa.