Rabbit P73 Polyclonal Antibody | anti-P73 antibody

P73 (Phospho-Tyr99)

Cat. Num. AAA191070

• Gene Names: TP73; P73
• Applications: Immunoblot, Western Blot
• Synonyms: P73; Polyclonal Antibody; P73 (Phospho-Tyr99); anti-P73 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous human p73 only when phosphorylated at tyrosine 99.
• Form/Format: 100ul at 1.0mg/ml in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Affinity-purified on phosphopeptide; non-phosphopeptide- reactive antibodies were removed by chromatography on non-phosphorylated peptide.
• Sequence Length: 587

• Applicable Applications for anti-P73 antibody: Immunoblotting (IB)
• Application Notes: Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~80kDa is detected.; Dilute in PBS or medium that is identical to that used in the assay system.
• Antigen: Peptide sequence that includes phosphorylation site of tyrosine 99 (S-P-Y(p)-A-Q) derived from human p73.
• Preparation and Storage: This antibody is stable for at least one (1) year at -20 degree C. Can be stored at 4 degree C for short-term.

Testing Data

Related Product Information for anti-P73 antibody

Tumor protein p73 is a member of the p53 family of transcription factors involved in cellular responses to stress and development. The family members include p53, p63, and p73 and have high sequence similarity to one another which allows p63 and p73 to transactivate p53- responsive genes causing cell cycle arrest and apoptosis. The family members can interact with each other in many ways involving direct or indirect protein interactions, resulting in regulation of the same target gene promoters or regulation of each other's promoters. The p73 protein is expressed at very low levels in normal tissues and is differentially expressed in a number of tumors.

NCBI and Uniprot Product Information

• NCBI GI #: 187828902
• NCBI GeneID: 7161
• NCBI Accession #: NP_001119712.1
• NCBI GenBank Nucleotide #: NM_001126240.2
• UniProt Accession #: O15350; O15351; Q17RN8; Q5TBV5; Q5TBV6; Q8NHW9; Q8TDY5; Q8TDY6; B7Z7J4; B7Z8Z1; B7Z9C1; C9J521
• Molecular Weight: 61,694 Da
• NCBI Official Full Name: tumor protein p73 isoform b
• NCBI Official Synonym Full Names: tumor protein p73
• NCBI Official Symbol: TP73
• NCBI Official Synonym Symbols: P73
• NCBI Protein Information: tumor protein p73
• UniProt Protein Name: Tumor protein p73
• UniProt Gene Name: TP73
• UniProt Synonym Gene Names: P73

NCBI Description

This gene encodes a member of the p53 family of transcription factors involved in cellular responses to stress and development. It maps to a region on chromosome 1p36 that is frequently deleted in neuroblastoma and other tumors, and thought to contain multiple tumor suppressor genes. The demonstration that this gene is monoallelically expressed (likely from the maternal allele), supports the notion that it is a candidate gene for neuroblastoma. Many transcript variants resulting from alternative splicing and/or use of alternate promoters have been found for this gene, but the biological validity and the full-length nature of some variants have not been determined. [provided by RefSeq, Feb 2011]

Uniprot Description

Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein.