Sheep Grb7 ELISA Kit | Grb7 elisa kit

Sheep Grb7 (Growth Factor Receptor Bound Protein 7) ELISA Kit

Cat. Num. AAA2501872

• Reactivity: Sheep
• Synonyms: Grb7; Sheep Grb7 (Growth Factor Receptor Bound Protein 7) ELISA Kit; Grb7 elisa kit

• Reactivity: Sheep
• Specificity: This kit recognizes natural and recombinant Sheep Grb7. No significant cross-reactivity or interference between Sheep Grb7 and analogues was observed.
• Sequence Length: 555

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 31.25-2000pg/mL
• Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Grb7 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Sheep Grb7 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Grb7. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Grb7 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Grb7, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Grb7. You can calculate the concentration of Grb7 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 334883159
• NCBI GeneID: 2886
• NCBI Accession #: NP_001229371.1
• NCBI GenBank Nucleotide #: NM_001242442.1
• UniProt Accession #: Q14451; Q53YD3; Q92568; Q96DF9; Q9Y220; B2RAV1; B3KNL0; B3KWP9; B7WP75; J3KQM4
• Molecular Weight: 55,920 Da
• NCBI Official Full Name: growth factor receptor-bound protein 7 isoform b
• NCBI Official Synonym Full Names: growth factor receptor-bound protein 7
• NCBI Official Symbol: GRB7
• NCBI Protein Information: growth factor receptor-bound protein 7; B47; GRB7 adapter protein; epidermal growth factor receptor GRB-7
• UniProt Protein Name: Growth factor receptor-bound protein 7
• Protein Family: Growth factor receptor-bound protein
• UniProt Gene Name: GRB7
• UniProt Entry Name: GRB7_HUMAN

NCBI Description

The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with epidermal growth factor receptor (EGFR) and ephrin receptors. The protein plays a role in the integrin signaling pathway and cell migration by binding with focal adhesion kinase (FAK). Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2011]

Uniprot Description

GRB7: Adapter protein that interacts with the cytoplasmic domain of numerous receptor kinases and modulates down-stream signaling. Promotes activation of down-stream protein kinases, including STAT3, AKT1, MAPK1 and/or MAPK3. Promotes activation of HRAS. Plays a role in signal transduction in response to EGF. Plays a role in the regulation of cell proliferation and cell migration. Plays a role in the assembly and stability of RNA stress granules. Binds to the 5'UTR of target mRNA molecules and represses translation of target mRNA species, when not phosphorylated. Phosphorylation impairs RNA binding and promotes stress granule disassembly during recovery after cellular stress. Homodimer. Interacts (via SH2 domain) with EGFR, ERBB2, ERBB3 (when phosphorylated), ERBB4 (when phosphorylated), EPHB1, INSR, FGFR1, PDGFRA (tyrosine phosphorylated) and PDGFRB (tyrosine phosphorylated). Interacts with SHC1. Interacts with RND1. Interacts (when tyrosine phosphorylated) with FHL2 and HAX1. Interacts (via SH2 domain) with RET and PTK2/FAK1. Interacts (when not phosphorylated) with ELAVL1. In stressed cells, but not in normal cells, part of a complex that contains at least GRB7, PTK2/FAK1, STAU1, ELAVL1 and TIA1. Interacts (via SH2 domain) with KIT (phosphorylated). Interacts (via SH2 domain) with TEK/TIE2 (tyrosine phosphorylated). Belongs to the GRB7/10/14 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: focal adhesion; cell projection; stress granule; plasma membrane; cytosol

Molecular Function: identical protein binding; protein binding; SH3/SH2 adaptor activity; RNA binding; protein kinase binding; phosphoinositide binding

Biological Process: epidermal growth factor receptor signaling pathway; stress granule assembly; positive regulation of signal transduction; negative regulation of translation; blood coagulation; leukocyte migration; positive regulation of cell migration