Mouse ASIC1 Monoclonal Antibody | anti-ASIC1 antibody

ASIC1 Monoclonal Antibody

Cat. Num. AAA190660

• Gene Names: ASIC1; ASIC; ACCN2; BNaC2
• Applications: Immunoblot, Western Blot
• Synonyms: ASIC1; Monoclonal Antibody; ASIC1 Monoclonal Antibody; Anti-AISC1; anti-ASIC1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: This antibody recognizes mouse and rat ASIC1. It does not cross-react with ASIC2.
• Sequence Length: 562

• Applicable Applications for anti-ASIC1 antibody: Immunoblot (IB)
• Application Notes: Immunoblotting: Use at 1ug/ml. A band of ~60kDa is detected.
• Antigen: Fusion protein corresponding to aa 460-526 (cytoplasmic C-terminus) of mouse ASIC1 (accession no. Q6NXK8).
• Positive Control: Rat brain lysate
• Dilution Instructions: Dilute in PBS or medium which is identical to that used in the assay system.
• Preparation and Storage: This antibody is stable for at least one (1) year at -2 0 degree C. Avoid repeated freezing and thawing.

Testing Data

Related Product Information for anti-ASIC1 antibody

Acid sensing (proton-gated) ion channel 1 (ASIC1) is a cation channel with high affinity for sodium which is gated by extracellular protons and inhibited by the diuretic amiloride. It is also permeable for Ca(2+), Li(+) and K(+) and mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. ASIC1 functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin-dependent protein kinase II phosphory-lation.

Product Categories/Family for anti-ASIC1 antibody

Ion Channel Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 378744190
• NCBI GeneID: 41
• NCBI Accession #: NP_001243759.1
• NCBI GenBank Nucleotide #: NM_001256830.1
• Molecular Weight: 62,700 Da
• NCBI Official Full Name: acid-sensing ion channel 1 isoform c
• NCBI Official Synonym Full Names: acid sensing ion channel subunit 1
• NCBI Official Symbol: ASIC1
• NCBI Official Synonym Symbols: ASIC; ACCN2; BNaC2
• NCBI Protein Information: acid-sensing ion channel 1
• UniProt Protein Name: Acid-sensing ion channel 1
• Protein Family: Acid-sensing ion channel
• UniProt Gene Name: ASIC1
• UniProt Synonym Gene Names: ACCN2; BNAC2; ASIC1; BNaC2
• UniProt Entry Name: ASIC1_HUMAN

NCBI Description

This gene encodes a member of the acid-sensing ion channel (ASIC) family of proteins, which are part of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. Members of the ASIC family are sensitive to amiloride and function in neurotransmission. The encoded proteins function in learning, pain transduction, touch sensation, and development of memory and fear. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2012]

Uniprot Description

ASIC1 iso1: non-voltage-gated amiloride-sensitive cation channel permeable for sodium, potassium and lithium. A member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. Expressed in most if not all brain neurons.

Protein type: Channel, cation; Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, ion channel

Chromosomal Location of Human Ortholog: 12q12

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: amiloride-sensitive sodium channel activity; protein binding

Biological Process: response to pH; sensory perception of sour taste; signal transduction; transport