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Mouse Hsp90alpha Monoclonal Antibody

Anti-Hsp90 alpha Monoclonal Antibody

Applications
Immunoblot, Western Blot, Immunoprecipitation
Synonyms
Hsp90alpha; Monoclonal Antibody; Anti-Hsp90 alpha Monoclonal Antibody; anti-Hsp90alpha antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Specificity
Hsp90alpha [Mouse monoclonal antibody recognizes Hsp90alpha of multiple species.]
This antibody recognizes human, mouse, rat, rabbit, bovine, porcine, and chicken Hsp90 alpha (90 kDa).
Form/Format
100ug in PBS, pH 7.4, purified by Protein G affinity chromatography.
Applicable Applications for anti-Hsp90alpha antibody
Immunoblot, Immunoprecipitation
Application Notes
Immunoblotting: use at 1-5ug/ml. A band of ~90 kDa is detected.
Immunoprecipitation: 5ug on 20ul Protein A-Sepharose + 100ul sample. These are recommended concentrations.
User should determine optimal concentrations for their application.
Positive control: Heat-shocked HeLa cell lysate.
Antigen
Full-length Hsp90 purified from chicken brain
Preservatives
0.09% sodium azide
Other Additives
50% glycerol
Dilutions Instructions
Dilute in PBS or medium which is identical to that used in the assay system.
Preparation and Storage
This antibody is stable for at least one (1) year at -20 degree C. Avoid repeated freezing and thawing.
Related Product Information for anti-Hsp90alpha antibody
Hsp90 and the 94 kDa glucose-regulated protein, Grp94, are major molecular chapeones of the cytosol and endoplasmic reticulum. In mammalian cells, there are at least two Hsp90 isoforms, Hsp90 alpha and Hsp90 beta , which are encoded by separate genes. All known members of the Hsp90 family are highly conserved, especially in the N-terminal and C-terminal regions. In the absence of stress, Hsp90 is an essential component of cellular processes such as hormone signaling and cell cycle control. Several regulatory proteins such as steroid receptors, cell cycle kinases and p53 have been identified as substrates of Hsp90.
Product Categories/Family for anti-Hsp90alpha antibody
References
Schuh S et al. 1985 J Biol Chem 260: 14292-14296; Sullivan WP et al. 1985 Biochemistry 24: 4214-4222.

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Product Notes

The Hsp90alpha (Catalog #AAA190475) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Hsp90alpha can be used in a range of immunoassay formats including, but not limited to, Immunoblot, Immunoprecipitation. Immunoblotting: use at 1-5ug/ml. A band of ~90 kDa is detected. Immunoprecipitation: 5ug on 20ul Protein A-Sepharose + 100ul sample. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Heat-shocked HeLa cell lysate. Researchers should empirically determine the suitability of the Hsp90alpha for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hsp90alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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