Mouse cdc34 Monoclonal Antibody | anti-CDC34 antibody

Cdc34 Monoclonal Antibody

Cat. Num. AAA190087

• Gene Names: CDC34; UBC3; UBCH3; UBE2R1; E2-CDC34
• Applications: Immunoblot, Western Blot, Immunoprecipitation
• Synonyms: cdc34; Monoclonal Antibody; Cdc34 Monoclonal Antibody; Anti-cdc34; Cdc34; anti-CDC34 antibody

• Host: Mouse
• Clonality: Monoclonal
• Specificity: Anti-cdc34 [Murine MAb to human Cdc34, a ubiquitin conjugating enzyme.]; This antibody recognizes human Cdc34.
• Form/Format: 100 ug in 10 mM PBS, pH 7.4.

• Applicable Applications for anti-CDC34 antibody: Immunoblot, Immunoprecipitation
• Application Notes: Immunoblotting and Immunoprecipitation: use at 1-5 ug/ml.; Positive controls: 293 cells and recombinant fusion protein.
• Antigen: N-terminus 6x-His tagged fusion protein encoding full-length human Cdc34 expressed in E. coli.
• Stabilizer: None
• Preservatives: None. Available on request
• Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.
• Preparation and Storage: This antibody is stable for at least one (1) year at -20 degree C. Avoid multiple freeze-thaw cycles.

Related Product Information for anti-CDC34 antibody

Cdc 34 is a ubiquitin conjugating enzyme that is essential for the transition from G1 to S phase in Saccharomyces cerevisiae. Human Cdc34 fully complements the budding yeast Cdc34 temperature sensitive mutant phenotype. In extracts of Xenopus laevis eggs, Cdc34 is required for the onset of DNA replication and appears to function in a multi-protein complex. Cdc34 is highly conserved among vertebrates and probably functions similarly in all eukaryotes to regulate the start of DNA synthesis.

Product Categories/Family for anti-CDC34 antibody

Neuroscience and Signal Transduction Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 18645180
• NCBI GeneID: 997
• UniProt Accession #: P49427; A8K689
• Molecular Weight: 26,737 Da
• NCBI Official Full Name: Cell division cycle 34 homolog (S. cerevisiae)
• NCBI Official Synonym Full Names: cell division cycle 34 homolog (S. cerevisiae)
• NCBI Official Symbol: CDC34
• NCBI Official Synonym Symbols: UBC3; UBCH3; UBE2R1; E2-CDC34
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 R1; ubiquitin carrier protein; ubiquitin-protein ligase R1; ubiquitin-conjugating enzyme E2-CDC34; ubiquitin-conjugating enzyme E2-32 KDA complementing
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 R1
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: CDC34
• UniProt Synonym Gene Names: UBCH3; UBE2R1
• UniProt Entry Name: UB2R1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication. [provided by RefSeq]

Uniprot Description

CDC34: a member of the ubiquitin-conjugating enzyme family. Is a part of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which is required for ubiquitin-mediated degradation of cell cycle G1 regulators. Required for the initiation of DNA replication.

Protein type: EC 6.3.2.19; Ligase; Ubiquitin ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; DNA replication initiation; positive regulation of neuron apoptosis; protein modification process; protein ubiquitination; G1/S transition of mitotic cell cycle