Fascin (FSCN) Recombinant Protein | FSCN recombinant protein

Recombinant Fascin (FSCN)

Cat. Num. AAA2009575

• Gene Names: Fscn1; Fan1; AI663989; fascin-1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Fascin (FSCN); Recombinant Fascin (FSCN); FSCN recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- EAFG FKVNASASSL KKKQIWTLEQ PPDEAGSAAV CLRSHLGRYL AADKDGNVTC EREVPDGDCR FLVVAHDDGR WSLQSEAHRR YFGGTEDRLS CFAQSVSPAE KWSVHIAMHP QVNIYSVTRK RYAHLSARPA DEIAVDRDVP WGVDSLITLA FQDQRYSVQT SDHRFLRHDG RLVARPEPAT GFTLEFRSGK VAFRDCEGRY LAPSGPSGTL KAGKATKVGK DELFALEQSC AQVVLQAANE RNV
• Sequence Length: 493

• Applicable Applications for FSCN recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Glu27~Val273 (Accession # Q61553) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 113680348
• NCBI GeneID: 14086
• NCBI Accession #: NP_032010.2
• NCBI GenBank Nucleotide #: NM_007984.2
• UniProt Accession #: Q61553; O09099; O09156; Q05DK3; Q7TN32; Q80V75
• Molecular Weight: 28.9kDa
• NCBI Official Full Name: fascin
• NCBI Official Synonym Full Names: fascin homolog 1, actin bundling protein (Strongylocentrotus purpuratus)
• NCBI Official Symbol: Fscn1
• NCBI Official Synonym Symbols: Fan1; AI663989; fascin-1
• NCBI Protein Information: fascin; fascin 1; singed-like protein
• UniProt Protein Name: Fascin
• UniProt Gene Name: Fscn1
• UniProt Synonym Gene Names: Fan1; Snl
• UniProt Entry Name: FSCN1_MOUSE

Uniprot Description

Fascin: Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration. Associates with beta-catenin. Ubiquitous. Belongs to the fascin family.

Protein type: Motility/polarity/chemotaxis; Actin-binding; Cytoskeletal

Cellular Component: microvillus; dendrite; actin cytoskeleton; ruffle; growth cone; cell projection; cytoskeleton; lamellipodium; cytoplasm; stress fiber; plasma membrane; cell junction; myelin sheath; filopodium

Molecular Function: actin filament binding; protein binding, bridging; protein binding; drug binding; actin binding

Biological Process: actin filament bundle formation; cell migration; cell motility involved in cell locomotion; actin filament organization; actin cytoskeleton organization and biogenesis