ELISA (EIA)
(Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human MSLN protein, mFc-His tagged protein can bind Rabbit anti-MSLN monoclonal antibody (clone: DM71) in a linear range of 1-100 ng/ml.)
Rabbit anti-Human mesothelin Monoclonal Antibody | anti-MSLN antibody
Anti-mesothelin antibody (DM71), Rabbit mAb
FC/FACS: 1:100
ELISA (EIA)
(Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human MSLN protein, mFc-His tagged protein can bind Rabbit anti-MSLN monoclonal antibody (clone: DM71) in a linear range of 1-100 ng/ml.)
ELISA (EIA)
(Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human MSLN protein, mFc-His tagged protein can bind Rabbit anti-MSLN monoclonal antibody (clone: DM71) in a linear range of 1-100 ng/ml.)
Testing Data
(Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human mesothelin (B) were surface stained with Rabbit anti-MSLN monoclonal antibody 1ug/ml (clone: DM71) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.)
Testing Data
(Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human mesothelin (B) were surface stained with Rabbit anti-MSLN monoclonal antibody 1ug/ml (clone: DM71) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.)
Flow Cytometry (FC/FACS)
(Figure 3. Flow cytometry data of serially titrated Rabbit anti-MSLN monoclonal antibody (clone: DM71) on Hela cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
Flow Cytometry (FC/FACS)
(Figure 3. Flow cytometry data of serially titrated Rabbit anti-MSLN monoclonal antibody (clone: DM71) on Hela cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
Testing Data
(Figure 4. Affinity ranking of different Rabbit anti-MSLN mAb clones by titration of different concentration onto Hela cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
Testing Data
(Figure 4. Affinity ranking of different Rabbit anti-MSLN mAb clones by titration of different concentration onto Hela cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]
Uniprot Description
MSLN: Membrane-anchored forms may play a role in cellular adhesion. Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer. Belongs to the mesothelin family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, GPI anchor; Cell adhesion
Chromosomal Location of Human Ortholog: 16p13.3
Cellular Component: Golgi apparatus; extracellular space; cell surface; membrane; plasma membrane
Molecular Function: protein binding
Biological Process: pancreas development; cell adhesion
Research Articles on MSLN
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Product Notes
The MSLN msln (Catalog #AAA188203) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-mesothelin antibody (DM71), Rabbit mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's mesothelin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS). ELISA: 1:5000-10,000 FC/FACS: 1:100. Researchers should empirically determine the suitability of the MSLN msln for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "mesothelin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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