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Flow Cytometry (FC/FACS) (Separation of Annexin positive Hoechst 34580 positive events (red-filled) from Annexin negative Hoechst 34580 negative events (black-dashed) in flow cytometry analysis (surface staining) of camptothecin induced apoptotic Jurkat cells stained using Annexin V Dyomics 647 (5 ul reagent per million cells in 100 ul of cell suspension).)

Annexin V Reagent

Annexin V Dyomics 647

Applications
Flow Cytometry, Functional Assay
Purity
Bacterially expressed annexin V is purified to homogeneity (>95% by HPLC) and conjugated under optimum conditions. The reagent is free of unconjugated fluorochrome.
Synonyms
Annexin V; Annexin V Dyomics 647; Annexin V reagent
Ordering
For Research Use Only!
Purity/Purification
Bacterially expressed annexin V is purified to homogeneity (>95% by HPLC) and conjugated under optimum conditions. The reagent is free of unconjugated fluorochrome.
Form/Format
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Applicable Applications for Annexin V reagent
Flow Cytometry (FC/FACS) (QC tested)
Conjugation
Dyomics 647
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Separation of Annexin positive Hoechst 34580 positive events (red-filled) from Annexin negative Hoechst 34580 negative events (black-dashed) in flow cytometry analysis (surface staining) of camptothecin induced apoptotic Jurkat cells stained using Annexin V Dyomics 647 (5 ul reagent per million cells in 100 ul of cell suspension).)

Flow Cytometry (FC/FACS) (Separation of Annexin positive Hoechst 34580 positive events (red-filled) from Annexin negative Hoechst 34580 negative events (black-dashed) in flow cytometry analysis (surface staining) of camptothecin induced apoptotic Jurkat cells stained using Annexin V Dyomics 647 (5 ul reagent per million cells in 100 ul of cell suspension).)

Flow Cytometry (FC/FACS)

(Flow cytometry multicolor surface staining of camptothecin induced apoptotic Jurkat cells stained using Annexin V Dyomics 647 (5 ul reagent per million cells in 100 ul of cell suspension) and Hoechst 34580 viability stain.)

Flow Cytometry (FC/FACS) (Flow cytometry multicolor surface staining of camptothecin induced apoptotic Jurkat cells stained using Annexin V Dyomics 647 (5 ul reagent per million cells in 100 ul of cell suspension) and Hoechst 34580 viability stain.)

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of camptothecin induced apoptotic Jurkat cells stained using Annexin V Dyomics 647 (5 ul reagent per million cells in 100 ul of cell suspension).)

Flow Cytometry (FC/FACS) (Flow cytometry surface staining pattern of camptothecin induced apoptotic Jurkat cells stained using Annexin V Dyomics 647 (5 ul reagent per million cells in 100 ul of cell suspension).)
Related Product Information for Annexin V reagent
Annexin V Dyomics 647 is intended for detection of early apoptotic cells by flow cytometry.

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Product Notes

The Annexin V (Catalog #AAA1802115) is a Reagent and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Annexin V can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS) (QC tested). Researchers should empirically determine the suitability of the Annexin V for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Annexin V, Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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