Mouse anti-Human HLA-G Monoclonal Antibody | anti-HLA-G antibody

Anti-HLA-G Alexa Fluor488

• Gene Names: HLA-G; MHC-G
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with Alexa Fluor488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: HLA-G; Monoclonal Antibody; Anti-HLA-G Alexa Fluor488; major histocompatibility complex; class I; G; anti-HLA-G antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone Number: 87G
• Specificity: The antibody 87G recognizes both membrane-bound and soluble forms of HLA-G (HLA-G1 and HLA-G5). HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells. The antibody 87G blocks interaction of HLA-G with inhibitory receptors.
• Purity/Purification: The purified antibody is conjugated with Alexa Fluor488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Phosphate buffered saline (PBS) solution with 15mM sodium azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-HLA-G antibody: Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: Extracellular and intracellular staining; recommended dilution: 1-3ug/ml; positive control: JEG-3 human choriocarcinoma epithelial cell line.
• Conjugation: Alexa Fluor488
• Negative Species: Mouse, Rat
• Immunogen: HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin.
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

References

*Sageshima N, Ishitani A, Omura M, Akasaki M, Umekage H, Katabuchi H, Okamura H, Hatake K: Necrotic feature of the trophoblasts lacking HLA-G expression in normal and pre-eclamptic placentas. Am J Reprod Immunol. 2003 Mar;49(3):174-82., URL: https://www.ncbi.nlm.nih.gov/pubmed/12797524dopt=,*Riteau B. et al.: HLA-G1 co-expression boosts the HLA class I-mediated NK lysis inhibition. Int Immunol. 13(2), 193 (2001)., URL: https://www.ncbi.nlm.nih.gov/pubmed/11157852dopt=,*Ishitani A. et al.: Protein Expression and Peptide Binding Suggest Unique and Interacting Functional Application (FUNC)tional Roles for HLA-E, F and G in Maternal-Placental Immune Recognitions. The Journal of Immunology 171, 1376 (2003)., URL: https://www.ncbi.nlm.nih.gov/pubmed/12874228dopt=,*Polakova K, Bandzuchova E, Hofmeister V, Weiss EH, Hutter H, Russ G: Binding analysis of HLA-G specific antibodies to hematopoietic cells isolated from leukemia patients. Neoplasma. 2003;50(5):331-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/14628085dopt=,*Polakova K, Krcova M, Kuba D, Russ G: Analysis of HLA-G expression in malignant hematopoetic cells from leukemia patients. Leuk Res. 2003 Jul;27(7):643-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/12681364dopt=,*Hackmon R, Hallak M, Krup M, Weitzman D, Sheiner E, Kaplan B, Weinstein Y: HLA-G antigen and parturition: maternal serum, fetal serum and amniotic fluid levels during pregnancy. Fetal Diagn Ther. 2004 Sep-Oct;19(5):404-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/15305096dopt=,*Rouas-Freiss N, Moreau P, Ferrone S, Carosella ED: HLA-G proteins in cancer: do they provide tumor cells with an escape mechanism Cancer Res. 2005 Nov 15;65(22):10139-44., URL: https://www.ncbi.nlm.nih.gov/pubmed/16287995dopt=,*Shobu T, Sageshima N, Tokui H, Omura M, Saito K, Nagatsuka Y, Nakanishi M, Hayashi Y, Hatake K, Ishitani A: The surface expression of HLA-F on decidual trophoblasts increases from mid to term gestation. J Reprod Immunol. 2006 Dec;72(1-2):18-32., URL: https://www.ncbi.nlm.nih.gov/pubmed/16806485dopt=,*LeMaoult J, Caumartin J, Daouya M, Favier B, Le Rond S, Gonzalez A, Carosella ED: Immune regulation by pretenders: cell-to-cell transfers of HLA-G make effector T cells act as regulatory cells. Blood. 2007 Mar 1;109(5):2040-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/17077329dopt=,*Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, Le Danff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N: Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum Immunol. 2003 Mar;64(3):315-26., URL: https://www.ncbi.nlm.nih.gov/pubmed/12590976dopt=,*Wiendl H: The non-classical MHC molecule HLA-G protects human muscle cells from immune-mediated lysis: implications for myoblast transplantation and gene therapy. Brain. 126(Pt 1), 176 (2003)., URL: https://www.ncbi.nlm.nih.gov/pubmed/12477705dopt=
*Creput C, Durrbach A, Menier C, Guettier C, Samuel D, Dausset J, Charpentier B, Carosella ED, Rouas-Freiss N. Human leukocyte antigen-G (HLA-G) expression in biliary epithelial cells is associated with allograft acceptance in liver-kidney transplantation. J Hepatol. 2003 39(4):587-94., URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgidb=p,*Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, LeDanff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N: Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum Immunol. 2003 64(3):315-26., URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgidb=p

NCBI and Uniprot Product Information

• NCBI GI #: 122132
• NCBI GeneID: 3135
• UniProt Accession #: P17693
• Molecular Weight: 338
• NCBI Official Full Name: HLA class I histocompatibility antigen, alpha chain G
• NCBI Official Synonym Full Names: major histocompatibility complex, class I, G
• NCBI Official Symbol: HLA-G
• NCBI Official Synonym Symbols: MHC-G
• NCBI Protein Information: HLA class I histocompatibility antigen, alpha chain G; HLA G antigen; b2 microglobulin; HLA class I molecule; MHC class I antigen G; HLA-G histocompatibility antigen, class I, G
• UniProt Protein Name: HLA class I histocompatibility antigen, alpha chain G
• Protein Family: HLA class I histocompatibility antigen
• UniProt Gene Name: HLA-G
• UniProt Synonym Gene Names: HLA-6.0; HLAG
• UniProt Entry Name: HLAG_HUMAN