Rat anti-Mouse CD25 Monoclonal Antibody | anti-CD25 antibody

Anti-Ms CD25 Purified

• Gene Names: Il2ra; CD25; Il2r; Ly-43
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation, Immunohistochemistry
• Purity: Purified by protein-A affinity chromatography
• Synonyms: CD25; Monoclonal Antibody; Anti-Ms CD25 Purified; IL-2R alpha chain; IL-2-RA; IL2-RA; p55; TAC antigen; IDDM10; interleukin 2 receptor subunit alpha; anti-CD25 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG1 lambda
• Clone Number: PC61.5
• Specificity: The rat monoclonal antibody PC61.5 (PC61.5.3) recognizes an extracellular epitope of CD25 (Interleukin-2 receptor alpha chain), a 55kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4⁺ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.
• Purity/Purification: Purified by protein-A affinity chromatography
• Form/Format: Purified. Phosphate buffered saline (PBS) solution with 15mM sodium azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD25 antibody: Flow Cytometry (FC/FACS) (QC tested), Immunoprecipitation (IP), Immunohistochemistry-Frozen (IHC-F)
• Application Notes: FC/FACS: Recommended dilution: 1-4ug/ml
• Immunogen: B6.1 CTL cell line
• Preparation and Storage: Store at 2-8 degree C. Do not freeze. Do not use after expiration date stamped on the label.

Related Product Information for anti-CD25 antibody

CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas.

References

*Tenorio EP, Olguín JE, Fernández J, Vieyra P, Saavedra R: Reduction of Foxp3+ cells by depletion with the PC61 mAb induces mortality in resistant BALB/c mice infected with Toxoplasma gondii. J Biomed Biotechnol. 2010;2010:786078., URL: http://www.ncbi.nlm.nih.gov/pubmed/20037737,*Wei HX, Chuang YH, Li B, Wei H, Sun R, Moritoki Y, Gershwin ME, Lian ZX, Tian Z: CD4+ CD25+ Foxp3+ regulatory T cells protect against T cell-mediated fulminant hepatitis in a TGF-beta-dependent manner in mice. J Immunol. 2008 Nov 15;181(10):7221-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/18981144,*Yu CT, Feng MH, Shih HM, Lai MZ: Increased p300 expression inhibits glucocorticoid receptor-T-cell receptor antagonism but does not affect thymocyte positive selection. Mol Cell Biol. 2002 Jul;22(13):4556-66., URL: https://www.ncbi.nlm.nih.gov/pubmed/12052865,*Aiba Y, Hirayama F, Ogawa M: Clonal proliferation and cytokine requirement of murine progenitors for natural killer cells. Blood. 1997 Jun 1;89(11):4005-12., URL: https://www.ncbi.nlm.nih.gov/pubmed/9166839,*Kish DD, Gorbachev AV, Fairchild RL: CD8+ T cells produce IL-2, which is required for CD(4+)CD25+ T cell regulation of effector CD8+ T cell development for contact hypersensitivity responses. J Leukoc Biol. 2005 Sep;78(3):725-35., URL: https://www.ncbi.nlm.nih.gov/pubmed/16000396,*Van VQ, Darwiche J, Raymond M, Lesage S, Bouguermouh S, Rubio M, Sarfati M: Cutting edge: CD47 controls the in vivo proliferation and homeostasis of peripheral CD4+ CD25+ Foxp3+ regulatory T cells that express CD103. J Immunol. 2008 Oct 15;181(8):5204-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/18832672,*Kotner J, Tarleton R: Endogenous CD4(+) CD25(+) regulatory T cells have a limited role in the control of Trypanosoma cruzi infection in mice. Infect Immun. 2007 Feb;75(2):861-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/17101658,*Jones CM, Cose SC, Coles RM, Winterhalter AC, Brooks AG, Heath WR, Carbone FR: Herpes simplex virus type 1-specific cytotoxic T-lymphocyte arming occurs within lymph nodes draining the site of cutaneous infection. J Virol. 2000 Mar;74(5):2414-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/10666272

NCBI and Uniprot Product Information

• NCBI GI #: 124318
• NCBI GeneID: 16184
• UniProt Accession #: P01590; Q61731
• Molecular Weight: 30,723 Da
• NCBI Official Full Name: Interleukin-2 receptor subunit alpha
• NCBI Official Synonym Full Names: interleukin 2 receptor, alpha chain
• NCBI Official Symbol: Il2ra
• NCBI Official Synonym Symbols: CD25; Il2r; Ly-43
• NCBI Protein Information: interleukin-2 receptor subunit alpha; IL2-RA; IL-2-RA; p55 chain; IL-2R alpha chain; IL-2R subunit alpha; IL-2 receptor subunit alpha
• UniProt Protein Name: Interleukin-2 receptor subunit alpha
• UniProt Gene Name: Il2ra
• UniProt Synonym Gene Names: Il2r; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA
• UniProt Entry Name: IL2RA_MOUSE

Uniprot Description

IL2RA: Receptor for interleukin-2. Non-covalent dimer of an alpha and a beta subunit. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit.

Protein type: Membrane protein, integral

Cellular Component: cell surface; membrane; integral to membrane; external side of plasma membrane

Molecular Function: protein binding; interleukin-2 receptor activity; drug binding; interleukin-2 binding

Biological Process: Notch signaling pathway; regulation of T cell homeostatic proliferation; negative regulation of immune response; negative regulation of defense response to virus; positive regulation of activated T cell proliferation; negative regulation of lymphocyte proliferation; inflammatory response to antigenic stimulus; negative regulation of T cell proliferation; positive regulation of T cell differentiation; negative regulation of inflammatory response; T cell homeostasis; positive regulation of T cell proliferation; immune response; activated T cell apoptosis