Mouse anti-Human SCIMP Monoclonal Antibody | anti-SCIMP antibody

Anti-SCIMP Purified

Cat. Num. AAA1801421

• Gene Names: SCIMP; UNQ5783; C17orf87
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot, Immunocytochemistry
• Purity: Purified by Protein-A Affinity Chromatography
• Synonyms: SCIMP; Monoclonal Antibody; Anti-SCIMP Purified; SLP adaptor and CSK interacting membrane protein; NVL; anti-SCIMP antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: NVL-07
• Specificity: The mouse monoclonal antibody NVL-07 recognizes intracellular part of human transmembrane adaptor SCIMP. This protein of 17 kDa predicted Mw migrates as a 22 kDa band on SDS PAGE.
• Purity/Purification: Purified by Protein-A Affinity Chromatography
• Form/Format: Phosphate buffered saline (PBS) solution with 15 mM sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 138

• Applicable Applications for anti-SCIMP antibody: Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB), Immunocytochemistry (ICC)
• Immunogen: Recombinant intracellular part of human SCIMP
• Preparation and Storage: Store at 2-8 degree C.

Flow Cytometry (FC/FACS)

(Flow cytometry analysis (intracellular staining) of SCIMP in a population of HEK-293T-SCIMP transfectants using monoclonal antibody (clone NVL-07, purified).The staining pattern reflects heterogeneity in the cell population regarding transfection efficiency.)

Related Product Information for anti-SCIMP antibody

SCIMP (SLP adaptor and Csk interacting membrane protein), also known as Nvl, is a palmitoylated transmembrane adaptor protein expressed in professional antigen presenting cells, most prominently in the lymph nodes and spleen. It is associated with tetraspanin-enriched microdomains (together with MHC II). There is a close relationship between SCIMP and tyrosinkinase Lyn, which is constitutively bound to it by its SH3 domain. After MHC II-mediated stimulation in the immunological synapse SCIMP becomes phosphorylated at several tyrosine residues and provides docking sites for Grb2 and SLP65 or SLP76 adaptors transducing the signal downstream, as well as for the kinase Csk with modulatory roles.

NCBI and Uniprot Product Information

• NCBI GI #: 427197601
• NCBI GeneID: 388325
• NCBI Accession #: NP_001258771.1
• NCBI GenBank Nucleotide #: NP_001258771.1
• UniProt Accession #: Q6UWF3
• NCBI Official Full Name: SLP adapter and CSK-interacting membrane protein isoform 2
• NCBI Official Synonym Full Names: SLP adaptor and CSK interacting membrane protein
• NCBI Official Symbol: SCIMP
• NCBI Official Synonym Symbols: UNQ5783; C17orf87
• NCBI Protein Information: SLP adapter and CSK-interacting membrane protein
• UniProt Protein Name: SLP adapter and CSK-interacting membrane protein
• Protein Family: SLP adapter and CSK-interacting membrane protein
• UniProt Gene Name: SCIMP
• UniProt Synonym Gene Names: C17orf87
• UniProt Entry Name: SCIMP_HUMAN

NCBI Description

This gene encodes a transmembrane adaptor protein that is expressed in antigen-presenting cells and is localized in the immunologic synapse. The encoded protein is involved in major histocompatibility complex class II signal transduction and immune synapse formation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2012]

Uniprot Description

UNQ5783: Lipid tetraspanin-associated transmembrane adapter/mediator involved in major histocompatibility complex (MHC) class II signaling transduction. Required in generating the calcium response and enhancing ERK activity upon MHC-II stimulation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 17p13.2

Cellular Component: membrane; integral to membrane; immunological synapse

Molecular Function: protein binding