Rabbit anti-Human SCIMP Polyclonal Antibody | anti-SCIMP antibody

SCIMP Antibody

Cat. Num. AAA7110267

• Gene Names: SCIMP; UNQ5783; C17orf87
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: SCIMP; Polyclonal Antibody; SCIMP Antibody; SLP adapter and CSK-interacting membrane protein; SLP65/SLP76; Csk-interacting membrane protein; C17orf87; UNQ5783/PRO16090; anti-SCIMP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 138

• Applicable Applications for anti-SCIMP antibody: ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: IHC: 1:500-1:1000; IF: 1:50-1:200
• Immunogen: Recombinant Human SLP adapter and CSK-interacting membrane protein (59-145AA)
• Conjugation: Non-conjugated
• Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunohistochemistry (IHC)

(IHC image of MBS7110267 diluted at 1:500 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunofluorescence (IF)

(Immunofluorescence staining of Hela cells with MBS7110267 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

Related Product Information for anti-SCIMP antibody

Lipid tetraspanin-associated transmembrane adapter/mediator involved in major histocompatibility complex (MHC) class II signaling transduction. Required in generating the calcium response and enhancing ERK activity upon MHC-II stimulation.

Product Categories/Family for anti-SCIMP antibody

Immunology; Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 427197601
• NCBI GeneID: 388325
• NCBI Accession #: NP_001258771.1
• NCBI GenBank Nucleotide #: NM_001271842.1
• UniProt Accession #: Q6UWF3; Q96MD0; A6XGL4; B4DLK1
• Molecular Weight: 15,693 Da
• NCBI Official Full Name: SLP adapter and CSK-interacting membrane protein isoform 2
• NCBI Official Synonym Full Names: SLP adaptor and CSK interacting membrane protein
• NCBI Official Symbol: SCIMP
• NCBI Official Synonym Symbols: UNQ5783; C17orf87
• NCBI Protein Information: SLP adapter and CSK-interacting membrane protein
• UniProt Protein Name: SLP adapter and CSK-interacting membrane protein
• Protein Family: SLP adapter and CSK-interacting membrane protein
• UniProt Gene Name: SCIMP
• UniProt Synonym Gene Names: C17orf87

NCBI Description

This gene encodes a transmembrane adaptor protein that is expressed in antigen-presenting cells and is localized in the immunologic synapse. The encoded protein is involved in major histocompatibility complex class II signal transduction and immune synapse formation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2012]

Uniprot Description

Lipid tetraspanin-associated transmembrane adapter/mediator involved in major histocompatibility complex (MHC) class II signaling transduction. Required in generating the calcium response and enhancing ERK activity upon MHC-II stimulation.