Western Blot (WB)
(Western blot analysis of WNK1 expression in rat testis extract (lane 1). WNK1 at 250KD was detected using rabbit anti- WNK1 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Rabbit WNK1 Polyclonal Antibody | anti-WNK1 antibody
Anti-WNK1 Antibody
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Immunohistochemisry Paraffin: 0.5-1mug/ml; Tested Species: Human, Mouse, Rat
Tested Species:In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Western Blot (WB)
(Western blot analysis of WNK1 expression in rat testis extract (lane 1). WNK1 at 250KD was detected using rabbit anti- WNK1 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Western Blot (WB)
(Western blot analysis of WNK1 expression in rat testis extract (lane 1). WNK1 at 250KD was detected using rabbit anti- WNK1 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Immunohistochemistry (IHC)
(WNK1 was detected in paraffin-embedded sections of mouse testis tissues using rabbit anti-WNK1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )
Immunohistochemistry (IHC)
(WNK1 was detected in paraffin-embedded sections of mouse testis tissues using rabbit anti-WNK1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )
Immunohistochemistry (IHC)
(WNK1 was detected in paraffin-embedded sections of rat testis tissues using rabbit anti-WNK1 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method. )
Immunohistochemistry (IHC)
(WNK1 was detected in paraffin-embedded sections of rat testis tissues using rabbit anti-WNK1 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method. )
Immunohistochemistry (IHC)
(WNK1 was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti-WNK1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )
Immunohistochemistry (IHC)
(WNK1 was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti-WNK1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )
Background: WNK lysine deficient protein kinase 1, also known as WNK1, is an enzyme that in humans is encoded by the WNK1 gene. The human gene is located on short arm of chromosome 12 (12p13.3). This gene encodes a member of the WNK subfamily of serine/threonine protein kinases. The encoded protein may be a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in this gene have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. Alternatively spliced transcript variants encoding different isoforms have been described but the full-length nature of all of them has yet to be determined.
2. Disse-Nicodème S, Achard JM, Desitter I, et al. (2000). "A new locus on chromosome 12p13.3 for pseudohypoaldosteronism type II, an autosomal dominant form of hypertension.". Am. J. Hum. Genet. 67 (2): 302-10.
3. Shekarabi M, Girard N, Rivière JB, et al. (July 2008). "Mutations in the nervous system--specific HSN2 exon of WNK1 cause hereditary sensory neuropathy type II". J Clin Invest. 118 (7): 2496-2505.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the WNK subfamily of serine/threonine protein kinases. The encoded protein may be a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in this gene have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. Alternatively spliced transcript variants encoding different isoforms have been described but the full-length nature of all of them has yet to be determined.[provided by RefSeq, May 2010]
Uniprot Description
WNK1: a serine-threonine protein kinase that controls sodium and chloride ion transport. May regulate the activity of the thiazide-sensitive Na-Cl cotransporter SLC12A3 by phosphorylation. May also play a role in actin cytoskeletal reorganization.
Protein type: Protein kinase, Other; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Other group; Wnk family
Chromosomal Location of Human Ortholog: 12p13.3
Cellular Component: cytoplasm; cytosol
Molecular Function: ATP binding; chloride channel inhibitor activity; phosphatase binding; protein binding; protein serine/threonine kinase activity
Biological Process: ion transport; peptidyl-threonine phosphorylation; positive regulation of ion transmembrane transporter activity; protein amino acid phosphorylation; regulation of cellular process
Disease: Neuropathy, Hereditary Sensory And Autonomic, Type Iia; Pseudohypoaldosteronism, Type Iic
Research Articles on WNK1
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Product Notes
The WNK1 wnk1 (Catalog #AAA178464) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-WNK1 Antibody reacts with Human, Mouse, Rat No cross reactivity with other proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's WNK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Western Blot: 0.1-0.5mug/ml; Tested Species: Rat; Predicted Species: Human Immunohistochemisry Paraffin: 0.5-1mug/ml; Tested Species: Human, Mouse, Rat Tested Species:In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Researchers should empirically determine the suitability of the WNK1 wnk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WNK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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