Mouse CCL1/TCA3 ELISA Kit | CCL1 elisa kit

Mouse CCL1/TCA3 PicoKine ELISA Kit

Cat. Num. AAA177382

• Gene Names: Ccl1; I-309; Scya1; Tca-3; BF534335
• Reactivity: Mouse
• Synonyms: CCL1/TCA3; Mouse CCL1/TCA3 PicoKine ELISA Kit; C-C motif chemokine 1; chemokine (C-C motif) ligand 1; CCL1; I-309inflammatory cytokine I-309; P500; SCYA1; SISe; small inducible cytokine A1 (I-309; homologous to mouse Tca-3); small-inducible cytokine A1; T lymphocyte-secreted protein I-309; TCA3; CCL1 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse CCL1
• Sequence Length: 92

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 7.8pg/ml-500pg/ml
• Sensitivity: < 1pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse CCL1/TCA3 PicoKine ELISA Kit standard curve )

Related Product Information for CCL1 elisa kit

Principle of the assay: mouse CCL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL1 has been precoated onto 96-well plates. Standards(NSO, K24-C92) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL1 amount of sample captured in plate.
Background: CCL1, Chemokine (C-C motif) ligand 1, is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine receptor CCR8.

NCBI and Uniprot Product Information

• NCBI GI #: 134512
• NCBI GeneID: 20290
• UniProt Accession #: P10146; P14098; Q9QUR9; Q9QUS5
• Molecular Weight: 9,836 Da
• NCBI Official Full Name: C-C motif chemokine 1
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 1
• NCBI Official Symbol: Ccl1
• NCBI Official Synonym Symbols: I-309; Scya1; Tca-3; BF534335
• NCBI Protein Information: C-C motif chemokine 1; P500; TCA3; CCR8 ligand; SIS-epsilon; T-cell activation protein 3; small inducible cytokine A1; small-inducible cytokine A1
• UniProt Protein Name: C-C motif chemokine 1
• Protein Family: Cyclin
• UniProt Gene Name: Ccl1
• UniProt Synonym Gene Names: Scya1; Tca3; TCA-3; TCA3
• UniProt Entry Name: CCL1_MOUSE

Uniprot Description

CCL1: Cytokine that is chemotactic for monocytes but not for neutrophils. Binds to CCR8. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Chemokine; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; chemokine activity; cytokine activity

Biological Process: immune response; chemotaxis