Human SLAM/CD150 ELISA Kit | SLAMF1 elisa kit

Human SLAM/CD150 PicoKine ELISA Kit

Cat. Num. AAA177273

• Gene Names: SLAMF1; SLAM; CD150; CDw150
• Reactivity: Human
• Synonyms: SLAM/CD150; Human SLAM/CD150 PicoKine ELISA Kit; Signaling lymphocytic activation molecule; signaling lymphocytic activation molecule family member 1; 4933415F16; AA177906; CD 150; Cd150; CD150 antigen; Cdw150; Estm51; Ipo 3; IPO-3; Ipo3; MGC151472; MGC151476; OTTHUMP00000025670; OTTHUMP00000060252; RGD1560634; Signaling lymphocytic activation molecule family member 1; isoform CRA_a; isoform CRA_b; Signaling lymphocytic activation molecule precursor; Slaf1; SLAF1_HUMAN; SLAM family; member 1; Slamf 1; Slamf1; SLAMF1 protein; SLAMF1 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human SLAM
• Sequence Length: 335

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human SLAM/CD150 PicoKine ELISA Kit standard curve )

Related Product Information for SLAMF1 elisa kit

Principle of the assay: human SLAM ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SLAM has been precoated onto 96-well plates. Standards(NSO, A21-K236) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SLAM is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human SLAM amount of sample captured in plate.
Background: Signaling lymphocytic activation molecule is a protein that in humans is encoded by the SLAMF1 gene. It belongs to the immunoglobulin gene superfamily. This gene is mapped to 1q23.3. It has found that SLAM is constitutively expressed on peripheral blood memory T cells, T-cell clones, immature thymocytes and a proportion of B cells, and is rapidly induced on naive T cells after activation. In MV-resistant cell lines, infection with clinical MV and expression of SLAM, but not CD46, caused cytopathic effects (CPE). The expression of SLAM on activated B and T lymphocytes correlates with the pathology of MV infection in humans and monkeys, in which lymphoid organs are the chief sites of MV replication and the binding of MV to SLAM may impair the signaling functions of SLAM in lymphocyte activation and inhibit Th0/Th1 cytokine production, thereby promoting Th2 cytokine production. It has reported that antibody-mediated ligation of SLAM on thymocytes triggered a protein tyrosine phosphorylation signal in T cells in a SAP-dependent manner. This signal also involved SHIP, the adaptor molecules DOK2, DOK1, and SHC and RASGAP.

NCBI and Uniprot Product Information

• NCBI GI #: 9297047
• NCBI GeneID: 6504
• UniProt Accession #: Q13291; Q5W172; Q9HBE8
• Molecular Weight: 33,839 Da
• NCBI Official Full Name: Signaling lymphocytic activation molecule
• NCBI Official Synonym Full Names: signaling lymphocytic activation molecule family member 1
• NCBI Official Symbol: SLAMF1
• NCBI Official Synonym Symbols: SLAM; CD150; CDw150
• NCBI Protein Information: signaling lymphocytic activation molecule; IPO-3
• UniProt Protein Name: Signaling lymphocytic activation molecule
• Protein Family: Signaling lymphocytic activation molecule
• UniProt Gene Name: SLAMF1
• UniProt Synonym Gene Names: SLAM
• UniProt Entry Name: SLAF1_HUMAN

Uniprot Description

SLAM: a type I membrane protein present on the surface of B cells, T cells and CD83+ dendritic cells. A high-affinity self-ligand considered to be important in bidirectional T <-> B-cell stimulation. SLAM-induced signal-transduction events in T lymphocytes are different from those in B cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A (SAP) acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (SHP-2)-dependent signal transduction operates. Its cytoplasmic domain interacts with SAP through part of its SH2 domain, and with SHP-2. Three alternatively spliced isoforms have been described.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: integral to membrane; phagocytic vesicle; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; antigen binding

Biological Process: viral reproduction; regulation of catalytic activity; positive regulation of cell proliferation; regulation of vesicle fusion; lymphocyte activation; signal transduction