SLAM/CD150 Active Protein | SLAMF1 active protein

SLAM/CD150 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120510

• Gene Names: SLAMF1; SLAM; CD150; CDw150
• Purity: >97% as determined by SDS-PAGE
• Synonyms: SLAM/CD150; SLAM/CD150 Protein; Human; Recombinant (His Tag); Human CD150/SLAM/SLAMF1 Protein (His Tag); CD150 Protein; CDw150 Protein; SLAM Protein; signaling lymphocytic activation molecule family member 1; SLAMF1 active protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Pro258

• Species: Human
• Activity: Measured by its ability to bind biotinylated recombinant human SH2D1A in a functional ELISA.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ala 21
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human SLAM (NP_003028.1) precursor (Met 1-Pro 258) with a C-terminal polyhistidine tag was expressed.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for SLAMF1 active protein

Background: CD150/signaling lymphocytic activation molecule (SLAM) is a cell surface sialylated phosphoglycoprotein and belongs to the CD2 subset of the Ig superfamily of type I transmembrane glycoproteins. The CD150 receptor is expressed on thymocytes, activated and memory T cells, B cells, platelets, natural killer T cells, and mature dendritic cells, and is also detected on tumor cells of Hodgkin's lymphoma (HL) and diffuse large B-cell lymphoma with an activated B cell phenotype. Additionally, it is the immune cell receptor for measles virus (MV). As a self-ligand, CD150 performs diverse immunologic functions including T/B-cell costimulation, induction of interferon gamma (IFN-gamma) in Th1 T-cell clones, redirection of Th2 clones to a Th1 or Th0 phenotype, and inhibition of apoptosis in B cells. Furthermore, CD150 was shown to be the second receptor for measles virus in addition to CD46, and the distribution of SLAM on various cell lines is consistent with their susceptibility to clinical isolates of measles virus.

Product Categories/Family for SLAMF1 active protein

ITIM/ITAM Immunoreceptors and Related Molecules

References

Tatsuo H, et al. (2002) The morbillivirus receptor SLAM (CD150). Microbiol Immunol. 46(3): 135-42.Sidorenko SP, et al. (2003)The dual-function CD150 receptor subfamily: the viral attraction. Nat Immunol. 4(1): 19-24.Yurchenko MY, et al. (2010) CD150 regulates JNK1/2 activation in normal and Hodgkin's lymphoma B cells. Immunol Cell Biol. 88(5): 565-74.Leonard VH, et al. (2010) Measles virus selectively blind to signaling lymphocytic activation molecule (SLAM ; CD150) is attenuated and induces strong adaptive immune responses in rhesus monkeys. J Virol. 84(7): 3413-20.

NCBI and Uniprot Product Information

• NCBI GI #: 4506969
• NCBI GeneID: 6504
• NCBI Accession #: NP_003028.1
• NCBI GenBank Nucleotide #: NM_003037.3
• UniProt Accession #: Q13291; Q5W172; Q9HBE8
• Molecular Weight: 33,839 Da
• NCBI Official Full Name: signaling lymphocytic activation molecule
• NCBI Official Synonym Full Names: signaling lymphocytic activation molecule family member 1
• NCBI Official Symbol: SLAMF1
• NCBI Official Synonym Symbols: SLAM; CD150; CDw150
• NCBI Protein Information: signaling lymphocytic activation molecule; IPO-3
• UniProt Protein Name: Signaling lymphocytic activation molecule
• Protein Family: Signaling lymphocytic activation molecule
• UniProt Gene Name: SLAMF1
• UniProt Synonym Gene Names: SLAM
• UniProt Entry Name: SLAF1_HUMAN

Uniprot Description

SLAM: a type I membrane protein present on the surface of B cells, T cells and CD83+ dendritic cells. A high-affinity self-ligand considered to be important in bidirectional T <-> B-cell stimulation. SLAM-induced signal-transduction events in T lymphocytes are different from those in B cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A (SAP) acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (SHP-2)-dependent signal transduction operates. Its cytoplasmic domain interacts with SAP through part of its SH2 domain, and with SHP-2. Three alternatively spliced isoforms have been described.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: integral to membrane; phagocytic vesicle; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; antigen binding

Biological Process: viral reproduction; regulation of catalytic activity; positive regulation of cell proliferation; regulation of vesicle fusion; lymphocyte activation; signal transduction