Human DKK-3 ELISA Kit | DKK3 elisa kit

Human DKK-3 PicoKine ELISA Kit

Cat. Num. AAA177233

• Gene Names: DKK3; RIG; REIC
• Reactivity: Human
• Synonyms: DKK-3; Human DKK-3 PicoKine ELISA Kit; Dickkopf-related protein 3; dickkopf WNT signaling pathway inhibitor 3; Dickkopf 3; Dickkopf 3 homolog (Xenopus laevis); Dickkopf 3 homolog; Dickkopf homolog 3; Dickkopf related protein 3; Dickkopf relatedprotein 3 Precursor; Dickkopf WNT signaling pathway inhibitor 3; Dickkopf; Xenopus; homolog of; 3; Dickkopf-3; Dickkopf3; DKK 3; Dkk-3; DKK3; DKK3_HUMAN; hDkk 3; hDkk-3; hDkk3; Regulated in glioma; REIC; RIG; RIG-like 5-6; RIG-like 7-1; DKK3 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human DKK-3
• Sequence Length: 350

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human DKK-3 PicoKine ELISA Kit standard curve )

Related Product Information for DKK3 elisa kit

Principle of the assay: human DKK-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for DKK-3 has been precoated onto 96-well plates. Standards(NSO, A22-I350) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DKK-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human DKK-3 amount of sample captured in plate.
Background: Dickkopf-related protein 3 is a protein that in humans is encoded by the DKK3 gene. This gene encodes a protein that is a member of the dickkopf family. It is mapped to 11p15.3. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Members of the Dkk-related family display unique patterns of mRNA expression in human and mouse tissues, and are secreted when expressed in 293T cells. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

NCBI and Uniprot Product Information

• NCBI GI #: 311033372
• NCBI GeneID: 27122
• UniProt Accession #: Q9UBP4; Q9ULB7; A8K1I2; D3DQW1
• Molecular Weight: 38,390 Da
• NCBI Official Full Name: Dickkopf-related protein 3
• NCBI Official Synonym Full Names: dickkopf WNT signaling pathway inhibitor 3
• NCBI Official Symbol: DKK3
• NCBI Official Synonym Symbols: RIG; REIC
• NCBI Protein Information: dickkopf-related protein 3; dkk-3; hDkk-3; dickkopf-3; RIG-like 5-6; RIG-like 7-1; dickkopf 3 homolog; dickkopf homolog 3; regulated in glioma
• UniProt Protein Name: Dickkopf-related protein 3
• Protein Family: Dickkopf-related protein
• UniProt Gene Name: DKK3
• UniProt Synonym Gene Names: REIC; Dickkopf-3; Dkk-3; hDkk-3
• UniProt Entry Name: DKK3_HUMAN

NCBI Description

This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

DKK3: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Belongs to the dickkopf family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11p15.2

Cellular Component: extracellular space

Biological Process: anatomical structure morphogenesis; Wnt receptor signaling pathway; adrenal gland development; negative regulation of aldosterone biosynthetic process; negative regulation of transcription, DNA-dependent