Western Blot (WB)
(Western Blot analysis of cell lysates: Lane 1: H202 untreated HeLa cell lysates Lane 2: H202 treated HeLa cell lysates Lane 3: Reduced recombinant GAPDH Lane 4: Oxidized recombinant GAPDH)
Rabbit anti-Human GAPDH, SO3 Polyclonal Antibody
GAPDH, SO3 (Glyceraldehyde-3-phosphate Dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH, GAPD, CDABP0047, OK/SW-cl.12)
Reactivity
Human
Applications
Western Blot
Purity
SerumSerum
Synonyms
GAPDH; SO3; Polyclonal Antibody; SO3 (Glyceraldehyde-3-phosphate Dehydrogenase; Peptidyl-cysteine S-nitrosylase GAPDH; GAPD; CDABP0047; OK/SW-cl.12); Anti -GAPDH; anti-GAPDH; SO3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human GAPDH, SO3.
Purity/Purification
Serum
Serum
Serum
Form/Format
Supplied as a liquid in HEPES, 0.15M sodium chloride, 0.01% BSA, 0.03% sodium azide, 50% glycerol.
Applicable Applications for anti-GAPDH, SO3 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1:2000
Dilution: Western Blot: 1:2000
Immunogen
Sulfonylated peptide corresponding to the oxidation sensitive site sequence to human GADPH (KLH).
Cellular Localization
Cytoplasm and Membrane
Positive Control
HeLa cell treated with H2O2
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of cell lysates: Lane 1: H202 untreated HeLa cell lysates Lane 2: H202 treated HeLa cell lysates Lane 3: Reduced recombinant GAPDH Lane 4: Oxidized recombinant GAPDH)
Western Blot (WB)
(Western Blot analysis of cell lysates: Lane 1: H202 untreated HeLa cell lysates Lane 2: H202 treated HeLa cell lysates Lane 3: Reduced recombinant GAPDH Lane 4: Oxidized recombinant GAPDH)
Related Product Information for anti-GAPDH, SO3 antibody
Glyceraldehyde-3-phosphate dehydrogenase(GAPDH) is a catalytic enzyme commonly known to be involved in glycolysis. The enzyme exists as a tetramer of identical 37kDa subunits. GAPDH catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, this ubiquitously expressed enzyme also displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Other studies also provide evidence of GAPDH playing an essential part of the program of gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. On recent study, GAPDH has identified of the most oxidant sensitive cell proteins. Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway.
Product Categories/Family for anti-GAPDH, SO3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
glyceraldehyde-3-phosphate dehydrogenase
Similar Products
Product Notes
The GAPDH, SO3 (Catalog #AAA647867) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GAPDH, SO3 (Glyceraldehyde-3-phosphate Dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH, GAPD, CDABP0047, OK/SW-cl.12) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GAPDH, SO3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:2000. Researchers should empirically determine the suitability of the GAPDH, SO3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAPDH, SO3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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