Typical Testing Data/Standard Curve (for reference only)
(Mouse TNFSF18/GITRL PicoKine ELISA Kit standard curve )
Mouse TNFSF18/GITRL ELISA Kit | TNFSF18 elisa kit
Mouse TNFSF18/GITRL PicoKine ELISA Kit
Gene Names
Tnfsf18; Gitrl
Reactivity
Mouse
Synonyms
TNFSF18/GITRL; Mouse TNFSF18/GITRL PicoKine ELISA Kit; Tumor necrosis factor ligand superfamily member 18; tumor necrosis factor (ligand) superfamily; member 18; Activation inducible TNF related ligand; Activation-inducible TNF-related ligand; AITRL; GITRL; Glucocorticoid induced TNF related ligand; Glucocorticoid induced TNFR related protein ligand; Glucocorticoid-induced TNF-related ligand; hGITRL; MGC138237; TL6; TNF18_HUMAN; TNFSF18; Tumor necrosis factor (ligand) superfamily member 18; TNFSF18 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse TNFSF18
Sequence Length
173
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
31.2pg/ml-2000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Mouse TNFSF18/GITRL PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse TNFSF18/GITRL PicoKine ELISA Kit standard curve )
Related Product Information for TNFSF18 elisa kit
Principle of the assay: mouse TNFSF18 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFSF18 has been precoated onto 96-well plates. Standards(NSO, T47-S173) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFSF18 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFSF18 amount of sample captured in plate.
Background: Tumor necrosis factor ligand superfamily member 18, also known as AITRL or GITRL, is a protein that in humans is encoded by the TNFSF18 gene. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor(TNF) ligand family. It is mapped to 1q25.1. TNFSF18 is a ligand for receptor TNFRSF18/AITR/GITR. It has been shown to modulate T lymphocyte survival in peripheral tissues. This cytokine is also found to be expressed in endothelial cells, and is thought to be important for interaction between T lymphocytes and endothelial cells. TNFSF18-dependent modulation of tryptophan catabolism may represent an important mechanism of action of glucocorticoids, both physiologically and therapeutically.
Background: Tumor necrosis factor ligand superfamily member 18, also known as AITRL or GITRL, is a protein that in humans is encoded by the TNFSF18 gene. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor(TNF) ligand family. It is mapped to 1q25.1. TNFSF18 is a ligand for receptor TNFRSF18/AITR/GITR. It has been shown to modulate T lymphocyte survival in peripheral tissues. This cytokine is also found to be expressed in endothelial cells, and is thought to be important for interaction between T lymphocytes and endothelial cells. TNFSF18-dependent modulation of tryptophan catabolism may represent an important mechanism of action of glucocorticoids, both physiologically and therapeutically.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
19,732 Da
NCBI Official Full Name
Tumor necrosis factor ligand superfamily member 18
NCBI Official Synonym Full Names
tumor necrosis factor (ligand) superfamily, member 18
NCBI Official Symbol
Tnfsf18
NCBI Official Synonym Symbols
Gitrl
NCBI Protein Information
tumor necrosis factor ligand superfamily member 18; GITR ligand; glucocorticoid-induced TNF-related ligand; glucocorticoid-induced-tumor necrosis factor receptor ligand
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 18
Protein Family
UniProt Gene Name
Tnfsf18
UniProt Synonym Gene Names
Gitrl; GITRL
UniProt Entry Name
TNF18_MOUSE
Uniprot Description
TNFSF18: Cytokine that binds to TNFRSF18/AITR/GITR. Regulates T- cell responses. Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation. Important for interactions between activated T-lymphocytes and endothelial cells. Mediates activation of NF-kappa-B. Belongs to the tumor necrosis factor family.
Protein type: Membrane protein, integral; Cytokine; Apoptosis
Cellular Component: extracellular space; cell surface; membrane; plasma membrane; integral to membrane
Molecular Function: protein binding; cytokine activity; tumor necrosis factor receptor superfamily binding; tumor necrosis factor receptor binding
Biological Process: cell-cell signaling; tumor necrosis factor-mediated signaling pathway; immune system process; T cell proliferation during immune response; regulation of T cell proliferation; immune response; activation of NF-kappaB transcription factor
Research Articles on TNFSF18
Similar Products
Product Notes
The Mouse TNFSF18 tnfsf18 (Catalog #AAA177163) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177163 ELISA Kit recognizes Mouse TNFSF18. It is sometimes possible for the material contained within the vial of "TNFSF18/GITRL, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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