Typical Testing Data/Standard Curve (for reference only)
Human Inter Alpha-Globulin Inhibitor H1 (ITIH1) ELISA Kit | ITIH1 elisa kit
Human Inter Alpha-Globulin Inhibitor H1 (ITIH1) ELISA Kit
Gene Names
ITIH1; H1P; ITIH; SHAP; IATIH; IGHEP1; ITI-HC1
Reactivity
Human
Synonyms
Inter Alpha-Globulin Inhibitor H1 (ITIH1); Human Inter Alpha-Globulin Inhibitor H1 (ITIH1) ELISA Kit; H1P; IATIH; ITIH; IGHEP1; Serum-derived hyaluronan-associated protein; Inter-alpha-trypsin inhibitor complex component III; ITIH1 elisa kit
Reactivity
Human
Specificity
his assay has high sensitivity and excellent specificity for detection of ITIH1. No significant cross-reactivity or interference between ITIH1 and analogues was observed.
Sequence Length
623
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
< 0.069ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ITIH1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ITIH1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ITIH1 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ITIH1 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to ITIH1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to ITIH1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ITIH1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ITIH1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to ITIH1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to ITIH1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ITIH1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ITIH1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for ITIH1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
69,495 Da
NCBI Official Full Name
inter-alpha-trypsin inhibitor heavy chain H1 isoform c
NCBI Official Synonym Full Names
inter-alpha-trypsin inhibitor heavy chain 1
NCBI Official Symbol
ITIH1
NCBI Official Synonym Symbols
H1P; ITIH; SHAP; IATIH; IGHEP1; ITI-HC1
NCBI Protein Information
inter-alpha-trypsin inhibitor heavy chain H1
UniProt Protein Name
Inter-alpha-trypsin inhibitor heavy chain H1
Protein Family
UniProt Gene Name
ITIH1
UniProt Synonym Gene Names
IGHEP1; ITI heavy chain H1; ITI-HC1; Inter-alpha-inhibitor heavy chain 1; SHAP
UniProt Entry Name
ITIH1_HUMAN
NCBI Description
The protein encoded by this gene is the heavy chain of a serine protease inhibitor that may serve to carry hyaluronan in plasma. This gene is part of a cluster of similar genes on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
Uniprot Description
ITIH1: May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes. Belongs to the ITIH family.
Protein type: Secreted; Inhibitor; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 3p21.1
Cellular Component: extracellular region
Molecular Function: serine-type endopeptidase inhibitor activity; calcium ion binding
Biological Process: hyaluronan metabolic process
Research Articles on ITIH1
Similar Products
Product Notes
The Human ITIH1 itih1 (Catalog #AAA2021440) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2021440 ELISA Kit recognizes Human ITIH1. It is sometimes possible for the material contained within the vial of "Inter Alpha-Globulin Inhibitor H1 (ITIH1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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