Mouse IL-21 ELISA Kit | IL21 elisa kit

Mouse IL-21 PicoKine ELISA Kit

Cat. Num. AAA176533

• Gene Names: Il21; IL-21
• Reactivity: Mouse
• Synonyms: IL-21; Mouse IL-21 PicoKine ELISA Kit; Interleukin-21; interleukin 21; Cytokine CX2; DN 452A22.7; IL 21; Interleukin 21; Interleukin 21 isoform; Interleukin21; OTTMUSP00000007838; Za11; IL21 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse IL-21
• Sequence Length: 146

• Samples: Cell culture supernates and serum
• Assay Type: Sandwich
• Detection Range: 62.5pg/ml-4000pg/ml
• Sensitivity: < 10pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse IL-21 PicoKine ELISA Kit standard curve )

Related Product Information for IL21 elisa kit

Principle of the assay: mouse IL-21 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-21 has been precoated onto 96-well plates. Standards(E.coli,P25-S146) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-21 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-21 amount of sample captured in plate.
Background: Interleukin-21 is a protein that in humans is encoded by the IL21 gene. Interleukin 21 is a cytokine that has potent regulatory effects on cells of the immune system, including natural killer (NK) cells and cytotoxic T cells that can destroy virally infected or cancerous cells. By radiation hybrid analysis, the IL21 gene is mapped to chromosome 4q26-q27, near the IL2 gene. IL21 stimulates B-cell proliferation in an anti-CD40-dependent manner but inhibits proliferation stimulated by IL4 plus anti-IgM. The biologic effects of IL21 on NK cells differ between species, with human IL21 enhancing NK cell proliferation and murine IL21 inhibiting NK cell proliferation. IL21 is a cytokine highly expressed by mouse T(H)17 cells.

NCBI and Uniprot Product Information

• NCBI GI #: 81870292
• NCBI GeneID: 60505
• UniProt Accession #: Q5SUE2; Q9ES17
• Molecular Weight: 16,811 Da
• NCBI Official Full Name: Interleukin 21 (Il21 protein)
• NCBI Official Synonym Full Names: interleukin 21
• NCBI Official Symbol: Il21
• NCBI Official Synonym Symbols: IL-21
• NCBI Protein Information: interleukin-21
• UniProt Protein Name: Interleukin
• Protein Family: Interleukin
• UniProt Gene Name: Il21
• UniProt Entry Name: Q5SUE2_MOUSE

Uniprot Description

IL21: Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells. May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation. Belongs to the IL-15/IL-21 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Cell development/differentiation; Apoptosis; Cytokine

Cellular Component: extracellular space; extracellular region

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interleukin-2 receptor binding; cytokine activity

Biological Process: positive regulation of interleukin-17 production; positive regulation of natural killer cell cytokine production; cell maturation; B cell apoptosis; positive regulation of natural killer cell mediated cytotoxicity; positive regulation of interleukin-10 production; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of natural killer cell differentiation; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of cell proliferation; positive regulation of B cell proliferation; positive regulation of B cell differentiation; immune response; positive regulation of T cell proliferation; positive regulation of interferon-gamma biosynthetic process