Immunohistochemistry (IHC)
(Anti-NOX5 antibody, MBS176174, IHC(P)IHC(P): Human Mammary Cancer Tissue )
Rabbit anti-Human NOX5 Polyclonal Antibody | anti-NOX5 antibody
Anti-NOX5 antibody
IHC (Paraffin-embedded section): Concentration: 0.5-1ug/ml; Tested Species: Hu; Antigen Retrieval: By Heat.
ICC: Concentration: 0.5-1ug/ml; Tested Species: Hu
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections to 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Immunohistochemistry (IHC)
(Anti-NOX5 antibody, MBS176174, IHC(P)IHC(P): Human Mammary Cancer Tissue )
Immunohistochemistry (IHC)
(Anti-NOX5 antibody, MBS176174, IHC(P)IHC(P): Human Mammary Cancer Tissue )
Immunocytochemistry (ICC)
(Anti-NOX5 antibody, MBS176174, ICCICC: HELA Cell )
Immunocytochemistry (ICC)
(Anti-NOX5 antibody, MBS176174, ICCICC: HELA Cell )
Western Blot (WB)
(Anti-NOX5 antibody, MBS176174, Western blottingAll lanes: Anti (MBS176174) at 0.5ug/mlLane 1: 22RV1 Whole Cell Lysate at 40ugLane 2: PANC Whole Cell Lysate at 40ugLane 3: HELA Whole Cell Lysate at 40ugLane 4: SKOV Whole Cell Lysate at 40ugPredicted bind size: 86KDObserved bind size: 86KD)
Western Blot (WB)
(Anti-NOX5 antibody, MBS176174, Western blottingAll lanes: Anti (MBS176174) at 0.5ug/mlLane 1: 22RV1 Whole Cell Lysate at 40ugLane 2: PANC Whole Cell Lysate at 40ugLane 3: HELA Whole Cell Lysate at 40ugLane 4: SKOV Whole Cell Lysate at 40ugPredicted bind size: 86KDObserved bind size: 86KD)
Background: NOX5(Nadph Oxidase 5), also known as NOX5A or NOX5B, is a protein which in humans is encoded by the NOX5 gene. The International Radiation Hybrid Mapping Consortium mapped the NOX5 gene to chromosome 15. NOX5 is a novel NADPH oxidase that generates superoxide and functions as an H+ channel in a Ca2+-dependent manner. Banfi et al.(2001) found that, when heterologously expressed, NOX5 was quiescent in unstimulated cells. However, in response to elevations of the cytosolic Ca2+ concentration, it generated large amounts of superoxide. Using RT-PCR and Southern and Western blot analyses, Kamiguti et al.(2005) identified NOX5 as a flavin-containing Ca2+-dependent oxidase present in hairy leukemic cells(HC), but not normal B cells.
NCBI and Uniprot Product Information
NCBI Description
This gene is predominantly expressed in the testis and lymphocyte-rich areas of spleen and lymph nodes. It encodes a calcium-dependen NADPH oxidase that generates superoxide, and functions as a calcium-dependent proton channel that may regulate redox-dependent processes in lymphocytes and spermatozoa. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]
Uniprot Description
NOX5: Calcium-dependent NADPH oxidase that generates superoxide. Also functions as a calcium-dependent proton channel and may regulate redox-dependent processes in lymphocytes and spermatozoa. May play a role in cell growth and apoptosis. Isoform v2 and isoform v5 are involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contribute to endothelial response to thrombin. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Oxidoreductase; EC 1.6.3.-; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 15q23
Cellular Component: endoplasmic reticulum; integral to membrane
Molecular Function: protein binding; FAD binding; superoxide-generating NADPH oxidase activity; heme binding; calcium ion binding; hydrogen ion channel activity; NADP binding
Biological Process: cell proliferation; proton transport; regulation of fusion of sperm to egg plasma membrane; apoptosis; endothelial cell proliferation; cytokinesis; regulation of proton transport; angiogenesis; cytokine secretion; superoxide release
Research Articles on NOX5
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Product Notes
The NOX5 nox5 (Catalog #AAA176174) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NOX5 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NOX5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC). Western Blot: Concentration: 0.1-0.5ug/ml; Tested Species: Hu IHC (Paraffin-embedded section): Concentration: 0.5-1ug/ml; Tested Species: Hu; Antigen Retrieval: By Heat. ICC: Concentration: 0.5-1ug/ml; Tested Species: Hu Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections to 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections. Researchers should empirically determine the suitability of the NOX5 nox5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NOX5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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