Rabbit integrin beta 4 binding protein Polyclonal Antibody | anti-EIF6 antibody

Anti-integrin beta 4 binding protein antibody

Cat. Num. AAA176103

• Gene Names: EIF6; CAB; EIF3A; eIF-6; p27BBP; ITGB4BP; b(2)gcn; p27(BBP)
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: integrin beta 4 binding protein; Polyclonal Antibody; Anti-integrin beta 4 binding protein antibody; Eukaryotic translation initiation factor 6; eukaryotic translation initiation factor 6; b(2)gcn antibody; B(2)GCN homolog antibody; B4 integrin interactor antibody; Binding protein of beta-4 integrin antibody; CAB antibody; eIF-6 antibody; EIF3A antibody; EIF6 antibody; Eukaryotic translation initiation factor 3A antibody; Eukaryotic translation initiation factor 6 antibody; IF6_HUMAN antibody; ITGB4BP antibody; OK/SW-cl.27 antibody; p27 beta 4 integrin binding protein antibody; p27(BBP) antibody; p27BBP antibody; RP4-614O4.1 antibody; anti-EIF6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 245

• Applicable Applications for anti-EIF6 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human integrin beta 4 binding protein (82-96aa QHIRNSLPDTVQIRR), different from the related rat and mouse sequences by one amino acid.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-EIF6 antibody, MBS176103, Western blottingLane 1: Rat Liver Tissue LysateLane 2: Rat Kidney Tissue LysateLane 3: COLO320 Cell LysateLane 4: SW620 Cell LysateLane 5: HELA Cell LysateLane 6: 293T Cell LysateLane 7: HEPA Cell Lysate)

Immunohistochemistry (IHC)

(Anti-EIF6 antibody, MBS176103, IHC(P)IHC(P): Human Intestinal Cancer Tissue )

Immunohistochemistry (IHC)

(Anti-EIF6 antibody, MBS176103, IHC(F)IHC(F): Human Placenta Tissue )

Related Product Information for anti-EIF6 antibody

Description: Rabbit IgG polyclonal antibody for Eukaryotic translation initiation factor 6(EIF6) detection. Tested with WB, IHC-P, IHC-F in Human, Mouse, Rat.
Background: EIF6(Eukaryotic Translation Initiation Factor 6), also called EIF3A or ITGB4BP, is a human gene. By fluorescence in situ hybridization, Sanvito et al.(1998) mapped the ITGB4BP gene to 20q11.2. Ceci et al.(2003) demonstrated that the ribosomal 60S subunit is activated by release of EIF6. In the cytoplasm, EIF6 is bound to free 60S but not to 80S subunits. Furthermore, EIF6 interacts in the cytoplasm with RACK1, a receptor for activated protein kinase C. Gandin et al.(2008) demonstrated that mammalian eIF6 is required for efficient initiation of translation in vivo. Eif6-null mouse embryos were lethal at preimplantation. Heterozygous mice had 50% reduction of eIF6 levels in all tissues, and showed reduced mass of hepatic and adipose tissues due to a lower number of cells and to impaired G1/S cell cycle progression.

NCBI and Uniprot Product Information

• NCBI GI #: 3122258
• NCBI GeneID: 3692
• UniProt Accession #: P56537; Q6IBN8; Q96TD5; B7ZBG9
• Molecular Weight: 23,735 Da
• NCBI Official Full Name: Eukaryotic translation initiation factor 6 {ECO:0000255|HAMAP-Rule:MF_03132}
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 6
• NCBI Official Symbol: EIF6
• NCBI Official Synonym Symbols: CAB; EIF3A; eIF-6; p27BBP; ITGB4BP; b(2)gcn; p27(BBP)
• NCBI Protein Information: eukaryotic translation initiation factor 6 {ECO:0000255|HAMAP-Rule:MF_03132}; B4 integrin interactor; p27 beta-4 integrin-binding protein; eukaryotic translation initiation factor 3A
• UniProt Protein Name: Eukaryotic translation initiation factor 6
• Protein Family: Translation initiation factor
• UniProt Gene Name: EIF6
• UniProt Synonym Gene Names: eIF-6
• UniProt Entry Name: IF6_HUMAN

NCBI Description

Hemidesmosomes are structures which link the basal lamina to the intermediate filament cytoskeleton. An important functional component of hemidesmosomes is the integrin beta-4 subunit (ITGB4), a protein containing two fibronectin type III domains. The protein encoded by this gene binds to the fibronectin type III domains of ITGB4 and may help link ITGB4 to the intermediate filament cytoskeleton. The encoded protein, which is insoluble and found both in the nucleus and in the cytoplasm, can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits. Multiple non-protein coding transcript variants and variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]

Uniprot Description

EIF6: eukaryotic translation initiation factor 6. Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex.

Protein type: Translation initiation; Nucleolus; Translation

Chromosomal Location of Human Ortholog: 20q12

Cellular Component: nucleoplasm; cytoplasm; lamin filament; nucleolus; nucleus

Molecular Function: protein binding; translation initiation factor activity; ribosome binding; ribosomal large subunit binding

Biological Process: translational initiation; ribosomal large subunit biogenesis and assembly; mature ribosome assembly; ribosome export from nucleus