Typical Testing Data/Standard Curve (for reference only)
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Mouse MIP-2 ELISA Kit | CXCL2 elisa kit
Mouse MIP-2 PicoKine ELISA Kit
Gene Names
Cxcl2; GROb; Gro2; Mip2; Scyb; MIP-2; Scyb2; MIP-2a; Mgsa-b; CINC-2a
Reactivity
Mouse
Synonyms
MIP-2; Mouse MIP-2 PicoKine ELISA Kit; C-X-C motif chemokine 2; chemokine (C-X-C motif) ligand 2; Chemokine (C-X-C motif) ligand 2; CINC-2a; Cxcl2; CXCL2_HUMAN; Gro beta; Gro-beta; GRO-beta(5-73); GRO-beta-T; GRO2; GRO2 oncogene; GROb; Growth-regulated protein beta; Hematopoietic synergistic factor; HSF; Macrophage inflammatory protein 2-alpha; Melanoma growth stimulatory activity beta; MGSA beta; MGSA-b; MIP 2; MIP 2a; MIP2; MIP2-alpha; MIP2A; SB-251353; SCYB2; CXCL2 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse Cxcl2
Sequence Length
100
Samples
Cell culture supernatants, cell lysates, serum and plasma (heparin, edta)
Detection Range
15.6 pg/ml - 1,000 pg/ml
Sensitivity
<5 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for CXCL2 elisa kit
Principle of the Assay: The Picokine™ Mouse Cxcl2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Cxcl2 with a 96-well strip plate that is pre-coated with antibody specific for Cxcl2. The detection antibody is a biotinylated antibody specific for Cxcl2. The capture antibody is monoclonal antibody from rat and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Cxcl2 with immunogen: Expression system for standard: E.coli; Immunogen sequence: A28-N100. The kit is analytically validated with ready-to-use reagents. To measure Mouse Cxcl2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse Cxcl2 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Cxcl2 in the sample.
Background/Introduction: MIP is a member of the aquaporin family of membrane-bound water channels. MIP family proteins are thought to contain 6 TM domains. Sequence analysis suggests that the proteins may have arisen through tandem, intragenic duplication from an ancestral protein that contained 3 TM domains. Major intrinsic protein (MIP, also called MP26) is the predominant fiber cell membrane protein of the ocular lens. The major intrinsic protein (MIP) of the vertebrate eye lens is the first identified member of a sequence-related family of cell-membrane proteins that appears to have evolved by gene duplication. Several members of the MIP family transport water (aquaporins), glycerol and other small molecules in microbial, plant and animal cells. The standard used in this kit is recombinant mouse MIP-2 (A28-N100), consisting of 73 amino acids with the molecular mass of 8KDa.
Background/Introduction: MIP is a member of the aquaporin family of membrane-bound water channels. MIP family proteins are thought to contain 6 TM domains. Sequence analysis suggests that the proteins may have arisen through tandem, intragenic duplication from an ancestral protein that contained 3 TM domains. Major intrinsic protein (MIP, also called MP26) is the predominant fiber cell membrane protein of the ocular lens. The major intrinsic protein (MIP) of the vertebrate eye lens is the first identified member of a sequence-related family of cell-membrane proteins that appears to have evolved by gene duplication. Several members of the MIP family transport water (aquaporins), glycerol and other small molecules in microbial, plant and animal cells. The standard used in this kit is recombinant mouse MIP-2 (A28-N100), consisting of 73 amino acids with the molecular mass of 8KDa.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
10,621 Da
NCBI Official Full Name
C-X-C motif chemokine 2
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 2
NCBI Official Symbol
Cxcl2
NCBI Official Synonym Symbols
GROb; Gro2; Mip2; Scyb; MIP-2; Scyb2; MIP-2a; Mgsa-b; CINC-2a
NCBI Protein Information
C-X-C motif chemokine 2; macrophage inflammatory protein 2; small inducible cytokine subfamily, member 2
UniProt Protein Name
C-X-C motif chemokine 2
Protein Family
UniProt Gene Name
Cxcl2
UniProt Synonym Gene Names
Mip-2; Mip2; Scyb2; MIP2
UniProt Entry Name
CXCL2_MOUSE
Uniprot Description
Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst.
Research Articles on CXCL2
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Product Notes
The Mouse CXCL2 cxcl2 (Catalog #AAA175915) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA175915 ELISA Kit recognizes Mouse CXCL2. It is sometimes possible for the material contained within the vial of "MIP-2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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